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Izvorni znanstveni članak
Head and Neck Tumor Cells Exhibit Altered Proliferation upon Overexpression of nm23 Genes
Maja Herak Bosnar
orcid.org/0000-0002-8448-4713
; Ruđer Bošković Institute, Zagreb, Croatia
Klara Dubravčić
; Clinical Hospital »Rebro«, Zagreb, Croatia
Ružica Bago
; Ruđer Bošković Institute, Zagreb, Croatia
Jasminka Pavelić
; Ruđer Bošković Institute, Zagreb, Croatia
| Puni tekst: engleski, pdf (163 KB) | str. 183-189 | citiraj | |
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APA 6th Edition Herak Bosnar, M., Dubravčić, K., Bago, R. i Pavelić, J. (2008). Head and Neck Tumor Cells Exhibit Altered Proliferation upon Overexpression of nm23 Genes. Croatica Chemica Acta, 81 (1), 183-189. Preuzeto s https://hrcak.srce.hr/23446 MLA 8th Edition Herak Bosnar, Maja, et al. "Head and Neck Tumor Cells Exhibit Altered Proliferation upon Overexpression of nm23 Genes." Croatica Chemica Acta, vol. 81, br. 1, 2008, str. 183-189. https://hrcak.srce.hr/23446. Citirano 08.03.2021. Chicago 17th Edition Herak Bosnar, Maja, Klara Dubravčić, Ružica Bago i Jasminka Pavelić. "Head and Neck Tumor Cells Exhibit Altered Proliferation upon Overexpression of nm23 Genes." Croatica Chemica Acta 81, br. 1 (2008): 183-189. https://hrcak.srce.hr/23446 Harvard Herak Bosnar, M., et al. (2008). 'Head and Neck Tumor Cells Exhibit Altered Proliferation upon Overexpression of nm23 Genes', Croatica Chemica Acta, 81(1), str. 183-189. Preuzeto s: https://hrcak.srce.hr/23446 (Datum pristupa: 08.03.2021.) Vancouver Herak Bosnar M, Dubravčić K, Bago R, Pavelić J. Head and Neck Tumor Cells Exhibit Altered Proliferation upon Overexpression of nm23 Genes. Croatica Chemica Acta [Internet]. 2008 [pristupljeno 08.03.2021.];81(1):183-189. Dostupno na: https://hrcak.srce.hr/23446 IEEE M. Herak Bosnar, K. Dubravčić, R. Bago i J. Pavelić, "Head and Neck Tumor Cells Exhibit Altered Proliferation upon Overexpression of nm23 Genes", Croatica Chemica Acta, vol.81, br. 1, str. 183-189, 2008. [Online]. Dostupno na: https://hrcak.srce.hr/23446. [Citirano: 08.03.2021.] |
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Sažetak
nm23 was identified as a metastasis suppressor gene but is also appointed to a number of other biological functions. The goal of this study was to reveal the influence of ectopic expression of nm23-H1 and nm23-H2 on proliferation properties of head and neck tumor cells. The proliferation rate of transfected cells was evaluated using EGFP reporter system and flow cytometry. HEp-2 and CAL 33 cells transiently transfected with nm23 cDNA containing constructs exhibited enhanced proliferation. CAL 27 cells constitutively expressing GFP-Nm23-H2 protein, exhibited intense proliferation the first day after seeding, while the GFP-Nm23-H1 expressing clone started to proliferate after one-day lag period. The results on transiently transfected HEp-2 and CAL 33 cells generally confirmed previous findings connecting nm23 expression with altered proliferation of head and neck tumors. We speculate that the effects observed on stably transfected CAL 27 clones are due to their different attachment properties.
Ključne riječi
nm23; head and neck tumor cell lines; flow cytometry; green fluorescent protein
Hrčak ID: 23446
URI
https://hrcak.srce.hr/23446
Moj profil
Srce