Šumarski list, Vol. 132 No. 7-8, 2008.
Izvorni znanstveni članak
Dna Analysis of London Planetree of Tree Lined Walkways of the City of Sarajevo
Emira Hukić
; Šumarski fakultet u Sarajevu
Aikaterini Dounavi
; Forstliche Versuch und Forschungs anstalt Baden-Württemberg
Dalibor Ballian
; Šumarski fakultet u Sarajevu
Sažetak
Interspecific hybridization often blurs the species boundaries both in natural and artificial conditions. Thus a verification of identity based on morphological characters only is not sometimes fully reliable. Such an example is the case of Platanus x acerifolia and P. orientalis co-occurring in city tree line, parks and artificial stands. Therefore we carried out an analysis using molecular markers to distinguish tree individuals that could not be assigned either to P. x acerifolia or P. orientalis. The individuals of P. x acerifolia and P. orientalis originated from three mixed groups collected in Sarajevo area and a group of P. orientalis from Cyprus was used as a control group. The four cpDNA microsatelite primers (ccmp3, ccmp6, ccmp7, ccmp10), an ITS sequence of trnT-trnL region and a pair of 5S nDNA primers were used to distinguish Platanus sp. individuals. No polymorphism was detected for any of analyzed DNA sequences and therefore distinction between the plane trees could not be achieved. The lack of polymorphism might be explained by dominant mode of inheritance for targeted sequences that are maternally transferred in hybridization processes.
Ključne riječi
DNA; London planetree (Patanus x acerifolia); microsatelite
Hrčak ID:
26353
URI
Datum izdavanja:
31.8.2008.
Posjeta: 2.723 *