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Evaluation of DNA Damage in Radiotherapy-Treated Cancer Patients Using the Alkaline Comet Assay

Marija Gamulin
Vera Garaj-Vrhovac
Nevenka Kopjar

Puni tekst: engleski, pdf (191 KB) str. 837-845 preuzimanja: 632* citiraj
APA 6th Edition
Gamulin, M., Garaj-Vrhovac, V. i Kopjar, N. (2007). Evaluation of DNA Damage in Radiotherapy-Treated Cancer Patients Using the Alkaline Comet Assay. Collegium antropologicum, 31 (3), 837-845. Preuzeto s https://hrcak.srce.hr/26898
MLA 8th Edition
Gamulin, Marija, et al. "Evaluation of DNA Damage in Radiotherapy-Treated Cancer Patients Using the Alkaline Comet Assay." Collegium antropologicum, vol. 31, br. 3, 2007, str. 837-845. https://hrcak.srce.hr/26898. Citirano 22.11.2019.
Chicago 17th Edition
Gamulin, Marija, Vera Garaj-Vrhovac i Nevenka Kopjar. "Evaluation of DNA Damage in Radiotherapy-Treated Cancer Patients Using the Alkaline Comet Assay." Collegium antropologicum 31, br. 3 (2007): 837-845. https://hrcak.srce.hr/26898
Harvard
Gamulin, M., Garaj-Vrhovac, V., i Kopjar, N. (2007). 'Evaluation of DNA Damage in Radiotherapy-Treated Cancer Patients Using the Alkaline Comet Assay', Collegium antropologicum, 31(3), str. 837-845. Preuzeto s: https://hrcak.srce.hr/26898 (Datum pristupa: 22.11.2019.)
Vancouver
Gamulin M, Garaj-Vrhovac V, Kopjar N. Evaluation of DNA Damage in Radiotherapy-Treated Cancer Patients Using the Alkaline Comet Assay. Collegium antropologicum [Internet]. 2007 [pristupljeno 22.11.2019.];31(3):837-845. Dostupno na: https://hrcak.srce.hr/26898
IEEE
M. Gamulin, V. Garaj-Vrhovac i N. Kopjar, "Evaluation of DNA Damage in Radiotherapy-Treated Cancer Patients Using the Alkaline Comet Assay", Collegium antropologicum, vol.31, br. 3, str. 837-845, 2007. [Online]. Dostupno na: https://hrcak.srce.hr/26898. [Citirano: 22.11.2019.]

Sažetak
The aim of this study was to evaluate primary DNA damage and the dynamics of the repair of radiotherapy-induced
DNA lesions in non-target cells of cancer patients. This study included patients diagnosed with different solid tumors
who received radiotherapy. The levels of DNA damage were evaluated using the alkaline comet assay on peripheral blood
leukocytes. Altogether four blood samples per patient were collected: before and after receiving the first dose of radiotherapy,
in the middle of radiotherapy cycle, and after the last dose of radiotherapy. The results indicate that after the first radiation
dose significantly increased levels of DNA damage were recorded in almost all cancer patients compared to their
baseline values. Specific patterns of DNA damage were recorded in samples analyzed in the middle of radiotherapy and
after receiving the last dose, indicating the possibility of adaptive response in some patients. Our results indicate that
persistence of post-irradiation damage in peripheral blood leukocytes (and possibly in other non-target cells) of cancer
patients that are strong determinants for the secondary cancer risk. Moreover, the alkaline comet assay was confirmed as
a rapid and sensitive assay for the assessment of genome damage after in vivo irradiation.

Ključne riječi
cancer; comet assay; leukocytes; DNA damage and repair

Hrčak ID: 26898

URI
https://hrcak.srce.hr/26898

Posjeta: 805 *