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Original scientific paper

Comparison of morphological and RAPD markers in evaluation of red clover (Trifolium pratense L.) changes caused by natural selection

SONJA GRLJUŠIĆ ; Department of Forage Crops Breeding and Genetics, Agricultural Institute Osijek, Južno predgrađe 17, 31000 Osijek, Croatia
SNJEŽANA BOLARIĆ ; Department of Plant Breeding, Genetics and Biometrics, Faculty of Agriculture, University of Zagreb, Svetošimunska cesta 25, 10000 Zagreb, Croatia
SVETISLAV POPOVIĆ ; Department of Forage Crops Breeding and Genetics, Agricultural Institute Osijek, Južno predgrađe 17, 31000 Osijek, Croatia
TIHOMIR ČUPIĆ ; Department of Forage Crops Breeding and Genetics, Agricultural Institute Osijek, Južno predgrađe 17, 31000 Osijek, Croatia
MARIJANA TUCAK ; Department of Forage Crops Breeding and Genetics, Agricultural Institute Osijek, Južno predgrađe 17, 31000 Osijek, Croatia
VINKO KOZUMPLIK ; Department of Plant Breeding, Genetics and Biometrics, Faculty of Agriculture, University of Zagreb, Svetošimunska cesta 25, 10000 Zagreb, Croatia

Fulltext: english, pdf (133 KB) pages 237-242 downloads: 1.543* cite
APA 6th Edition
GRLJUŠIĆ, S., BOLARIĆ, S., POPOVIĆ, S., ČUPIĆ, T., TUCAK, M. & KOZUMPLIK, V. (2008). Comparison of morphological and RAPD markers in evaluation of red clover (Trifolium pratense L.) changes caused by natural selection. Periodicum biologorum, 110 (3), 237-242. Retrieved from https://hrcak.srce.hr/32572
MLA 8th Edition
GRLJUŠIĆ, SONJA, et al. "Comparison of morphological and RAPD markers in evaluation of red clover (Trifolium pratense L.) changes caused by natural selection." Periodicum biologorum, vol. 110, no. 3, 2008, pp. 237-242. https://hrcak.srce.hr/32572. Accessed 23 Oct. 2019.
Chicago 17th Edition
GRLJUŠIĆ, SONJA, SNJEŽANA BOLARIĆ, SVETISLAV POPOVIĆ, TIHOMIR ČUPIĆ, MARIJANA TUCAK and VINKO KOZUMPLIK. "Comparison of morphological and RAPD markers in evaluation of red clover (Trifolium pratense L.) changes caused by natural selection." Periodicum biologorum 110, no. 3 (2008): 237-242. https://hrcak.srce.hr/32572
Harvard
GRLJUŠIĆ, S., et al. (2008). 'Comparison of morphological and RAPD markers in evaluation of red clover (Trifolium pratense L.) changes caused by natural selection', Periodicum biologorum, 110(3), pp. 237-242. Available at: https://hrcak.srce.hr/32572 (Accessed 23 October 2019)
Vancouver
GRLJUŠIĆ S, BOLARIĆ S, POPOVIĆ S, ČUPIĆ T, TUCAK M, KOZUMPLIK V. Comparison of morphological and RAPD markers in evaluation of red clover (Trifolium pratense L.) changes caused by natural selection. Periodicum biologorum [Internet]. 2008 [cited 2019 October 23];110(3):237-242. Available from: https://hrcak.srce.hr/32572
IEEE
S. GRLJUŠIĆ, S. BOLARIĆ, S. POPOVIĆ, T. ČUPIĆ, M. TUCAK and V. KOZUMPLIK, "Comparison of morphological and RAPD markers in evaluation of red clover (Trifolium pratense L.) changes caused by natural selection", Periodicum biologorum, vol.110, no. 3, pp. 237-242, 2008. [Online]. Available: https://hrcak.srce.hr/32572. [Accessed: 23 October 2019]

Abstracts
Abstract

Background and Purpose: Accurate assessment of the levels and patterns of genetic variability and identification of differences resulting from natural selection can be invaluable in red clover breeding for diverse applications. The study was conducted to compare the efficiency of morphological and random amplified polymorphic DNA (RAPD) markers in evaluation of changes in red clover (Trifolium pratense L.) cultivars caused by natural selection.

Materials and Methods: Three red clover cultivars and their selections, which had survived for three years growing in an upland region, provided the basic material. Morphological and RAPD data were analyzed by NTSYS-pc software. The number of markers detected, values and ranges of distances calculated and 2D Principal Coordinate Analyses (2D PCoA) based on both marker techniques were compared.

Results: Although both marker methods indicated differences among cultivars and selections, parameters differed in values. The number of loci detected by RAPD markers was much higher than that determined with morphological traits. The values and ranges of morphological distances were higher and wider than those of molecular distances. The eigenvectors of 2D PCoA based on morphological data accounted for more of the total variation observed in the whole data set.

Conclusions: Each of the data sets has its own strengths and constraints. For reasonably accurate estimates of genetic variability and its changes under natural selection, adequate attention has to be devoted to both marker methods.

Keywords
Trifolium pratense L.; morphological traits; RAPD markers

Hrčak ID: 32572

URI
https://hrcak.srce.hr/32572

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