Izvorni znanstveni članak
Optimization of Carbon and Nitrogen Sources for L-asparaginase Production by Enterobacter aerogenes using Response Surface Methodology
G. Baskar
orcid.org/0000-0003-1061-421X
; Department of Biotechnology, St. Joseph’s College of Engineering, Chennai - 600119, India
M. Dharmendira Kumar
; Department of Chemical Engineering, Alagappa College of Technology, Anna University, Chennai - 600025, India
A. Anand Prabu
; Green Energy Center for Environmental Studies, College of Environmental and Applied Chemistry, Kyung Hee University, Gyeonggi-do, 446-701, South Korea
S. Renganathan
; Department of Chemical Engineering, Alagappa College of Technology, Anna University, Chennai - 600025, India
ChangKyoo Yoo
; Green Energy Center for Environmental Studies, College of Environmental and Applied Chemistry, Kyung Hee University, Gyeonggi-do, 446-701, South Korea
Sažetak
A full factorial central composite design (CCD) was applied to study various effects of sodium citrate, diammonium hydrogen phosphate (DAHP) and L-asparagine to determine the optimal concentration (γ) of these compounds on L-asparaginase production by Enterobacter aerogenes MTCC 2823 under shake flask fermentation conditions. A second order polynomial model describing the relationship between the variables and the L-asparaginase activity was fitted in coded units of variables. The statistical reliability and significance of the model was validated by F-test for analysis of variance at higher R2
value (R2 = 0.871). The optimum estimated concentration of sodium citrate (X1), DAHP (X2) and L-asparagine (X3) was 18.76, 5.72 and 8.58 g L–1 respectively with maximum L-asparaginase activity of 19.129 IU mL–1. The composite desirability of 98.38 % reveals
the validity of the model and predicted values. The L-asparaginase activity was increased by 5.96 % than predicted activity, after optimization of carbon and nitrogen sources for L-asparaginase production by Enterobacter aerogenes MTCC 2823 using CCD.
Ključne riječi
Fermentation; optimization; polynomial model; response surface plots; carbon and nitrogen sources
Hrčak ID:
40936
URI
Datum izdavanja:
29.9.2009.
Posjeta: 1.297 *