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Assessment of the Radioprotective Effects of Amifostine and Melatonin on Human Lymphocytes Irradiated with Gamma-Rays In Vitro

Nevenka Kopjar
Slavica Miočić
Snježana Ramić
Mirta Milić
Tomislav Viculin

Puni tekst: engleski, pdf (170 KB) str. 155-163 preuzimanja: 1.262* citiraj
APA 6th Edition
Kopjar, N., Miočić, S., Ramić, S., Milić, M. i Viculin, T. (2006). Assessment of the Radioprotective Effects of Amifostine and Melatonin on Human Lymphocytes Irradiated with Gamma-Rays In Vitro. Arhiv za higijenu rada i toksikologiju, 57 (2), 155-163. Preuzeto s https://hrcak.srce.hr/3919
MLA 8th Edition
Kopjar, Nevenka, et al. "Assessment of the Radioprotective Effects of Amifostine and Melatonin on Human Lymphocytes Irradiated with Gamma-Rays In Vitro." Arhiv za higijenu rada i toksikologiju, vol. 57, br. 2, 2006, str. 155-163. https://hrcak.srce.hr/3919. Citirano 24.06.2019.
Chicago 17th Edition
Kopjar, Nevenka, Slavica Miočić, Snježana Ramić, Mirta Milić i Tomislav Viculin. "Assessment of the Radioprotective Effects of Amifostine and Melatonin on Human Lymphocytes Irradiated with Gamma-Rays In Vitro." Arhiv za higijenu rada i toksikologiju 57, br. 2 (2006): 155-163. https://hrcak.srce.hr/3919
Harvard
Kopjar, N., et al. (2006). 'Assessment of the Radioprotective Effects of Amifostine and Melatonin on Human Lymphocytes Irradiated with Gamma-Rays In Vitro', Arhiv za higijenu rada i toksikologiju, 57(2), str. 155-163. Preuzeto s: https://hrcak.srce.hr/3919 (Datum pristupa: 24.06.2019.)
Vancouver
Kopjar N, Miočić S, Ramić S, Milić M, Viculin T. Assessment of the Radioprotective Effects of Amifostine and Melatonin on Human Lymphocytes Irradiated with Gamma-Rays In Vitro. Arh Hig Rada Toksikol. [Internet]. 2006 [pristupljeno 24.06.2019.];57(2):155-163. Dostupno na: https://hrcak.srce.hr/3919
IEEE
N. Kopjar, S. Miočić, S. Ramić, M. Milić i T. Viculin, "Assessment of the Radioprotective Effects of Amifostine and Melatonin on Human Lymphocytes Irradiated with Gamma-Rays In Vitro", Arhiv za higijenu rada i toksikologiju, vol.57, br. 2, str. 155-163, 2006. [Online]. Dostupno na: https://hrcak.srce.hr/3919. [Citirano: 24.06.2019.]

Sažetak
Radioprotective effects of amifostine and melatonin as well as their ability to modulate the level of spontaneous and gamma-irradiation-induced genetic changes on human peripheral blood lymphocytes were investigated using the cytokinesis-block micronucleus (CBMN) assay and sister chromatid exchange (SCE). Parallel blood samples were pre-treated with amifostine, melatonin and their combination for 30 minutes. Negative controls were also included. After the treatment with radioprotectors, one blood sample of each experimental group was exposed to gamma-rays from a 60Co source. The radiation dose absorbed was 2 Gy. Our research confirmed the radioprotective effects of both chemicals in vitro, with no significant genotoxicity. Pre-treated irradiated blood samples showed a decrease in the total number of micronuclei (MN) and in the number of cells with more than one MN. They also showed significantly lower mean SCE values. This study shows that it is possible combine these radioprotectors by adjusting the doses of amifostine to achieve the best radioprotective effect with as few side effects as possible. However, further in vitro and clinical studies are needed to clarify their mechanisms of action and possible interactions.

Ključne riječi
amifostine; gamma-rays; human lymphocytes; ionising radiation; melatonin; peripheral blood; radioprotectors

Hrčak ID: 3919

URI
https://hrcak.srce.hr/3919

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