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Endotoxin Measurement in House Dust Using the End-point Limulus Amoebocyte Lysate Method

Veda Marija Varnai
Jelena Macan
Davor Plavec
Dijana Jureša

Puni tekst: engleski, pdf (238 KB) str. 175-181 preuzimanja: 601* citiraj
APA 6th Edition
Varnai, V.M., Macan, J., Plavec, D. i Jureša, D. (2004). Endotoxin Measurement in House Dust Using the End-point Limulus Amoebocyte Lysate Method. Arhiv za higijenu rada i toksikologiju, 55 (2-3), 175-181. Preuzeto s https://hrcak.srce.hr/308
MLA 8th Edition
Varnai, Veda Marija, et al. "Endotoxin Measurement in House Dust Using the End-point Limulus Amoebocyte Lysate Method." Arhiv za higijenu rada i toksikologiju, vol. 55, br. 2-3, 2004, str. 175-181. https://hrcak.srce.hr/308. Citirano 20.09.2020.
Chicago 17th Edition
Varnai, Veda Marija, Jelena Macan, Davor Plavec i Dijana Jureša. "Endotoxin Measurement in House Dust Using the End-point Limulus Amoebocyte Lysate Method." Arhiv za higijenu rada i toksikologiju 55, br. 2-3 (2004): 175-181. https://hrcak.srce.hr/308
Harvard
Varnai, V.M., et al. (2004). 'Endotoxin Measurement in House Dust Using the End-point Limulus Amoebocyte Lysate Method', Arhiv za higijenu rada i toksikologiju, 55(2-3), str. 175-181. Preuzeto s: https://hrcak.srce.hr/308 (Datum pristupa: 20.09.2020.)
Vancouver
Varnai VM, Macan J, Plavec D, Jureša D. Endotoxin Measurement in House Dust Using the End-point Limulus Amoebocyte Lysate Method. Arh Hig Rada Toksikol. [Internet]. 2004 [pristupljeno 20.09.2020.];55(2-3):175-181. Dostupno na: https://hrcak.srce.hr/308
IEEE
V.M. Varnai, J. Macan, D. Plavec i D. Jureša, "Endotoxin Measurement in House Dust Using the End-point Limulus Amoebocyte Lysate Method", Arhiv za higijenu rada i toksikologiju, vol.55, br. 2-3, str. 175-181, 2004. [Online]. Dostupno na: https://hrcak.srce.hr/308. [Citirano: 20.09.2020.]

Sažetak
Endotoxin is a lipopolysaccharide, a part of Gram-negative bacteria cell membrane commonly present in general and many occupational environments. This paper describes sample preparation and endotoxin measurement in 16 samples of house dust from urban homes (Zagreb, Croatia) using end-point chromogenic Limulus amoebocyte lysate (LAL) bioassay. House dust was collected on cellulose filters by vacuuming bedroom and living room floors, and was kept frozen until assayed. Samples were extracted from filters with a 0.05 % solution of Tween-20 in endotoxin-free water. Serial dilutions of samples were measured in duplicates. The linearity of the standard curve was satisfying (r=0.983), as well as the recovery (92 and 110 %) and repeatability (coefficient of variation from 0 to 8.5 %). The endotoxin levels found in the house dust samples ranged from 4.8 to 200 EU/mg, with the arithmetic mean of 49.5 EU/mg (standard error of the mean of 12.1 EU/mg), and were in the range of house dust endotoxin values obtained by other authors.

Ključne riječi
endotoxin; LAL assay; lipopolysaccharide; Limulus polyphemus

Hrčak ID: 308

URI
https://hrcak.srce.hr/308

[hrvatski]

Posjeta: 1.130 *