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Acta Pharmaceutica, Vol. 56 No. 2, 2006.

Izvorni znanstveni članak

Novel colorimetric assay of indomethacin using 4-carboxyl-2,6-dinitrobenzene diazonium ion

OLAJIRE AREMU ADEGOKE
OLAKUNLE SUNDAY IDOWU
AJIBOLA AKINYEMI OLANIYI

Puni tekst: engleski, pdf (240 KB) str. 189-202 preuzimanja: 1.712* citiraj
APA 6th Edition
ADEGOKE, O.A., IDOWU, O.S. i OLANIYI, A.A. (2006). Novel colorimetric assay of indomethacin using 4-carboxyl-2,6-dinitrobenzene diazonium ion. Acta Pharmaceutica, 56 (2), 189-202. Preuzeto s https://hrcak.srce.hr/4555
MLA 8th Edition
ADEGOKE, OLAJIRE AREMU, et al. "Novel colorimetric assay of indomethacin using 4-carboxyl-2,6-dinitrobenzene diazonium ion." Acta Pharmaceutica, vol. 56, br. 2, 2006, str. 189-202. https://hrcak.srce.hr/4555. Citirano 17.02.2019.
Chicago 17th Edition
ADEGOKE, OLAJIRE AREMU, OLAKUNLE SUNDAY IDOWU i AJIBOLA AKINYEMI OLANIYI. "Novel colorimetric assay of indomethacin using 4-carboxyl-2,6-dinitrobenzene diazonium ion." Acta Pharmaceutica 56, br. 2 (2006): 189-202. https://hrcak.srce.hr/4555
Harvard
ADEGOKE, O.A., IDOWU, O.S., i OLANIYI, A.A. (2006). 'Novel colorimetric assay of indomethacin using 4-carboxyl-2,6-dinitrobenzene diazonium ion', Acta Pharmaceutica, 56(2), str. 189-202. Preuzeto s: https://hrcak.srce.hr/4555 (Datum pristupa: 17.02.2019.)
Vancouver
ADEGOKE OA, IDOWU OS, OLANIYI AA. Novel colorimetric assay of indomethacin using 4-carboxyl-2,6-dinitrobenzene diazonium ion. Acta Pharm. [Internet]. 2006 [pristupljeno 17.02.2019.];56(2):189-202. Dostupno na: https://hrcak.srce.hr/4555
IEEE
O.A. ADEGOKE, O.S. IDOWU i A.A. OLANIYI, "Novel colorimetric assay of indomethacin using 4-carboxyl-2,6-dinitrobenzene diazonium ion", Acta Pharmaceutica, vol.56, br. 2, str. 189-202, 2006. [Online]. Dostupno na: https://hrcak.srce.hr/4555. [Citirano: 17.02.2019.]

Sažetak
A simple, sensitive and direct colorimetric method for the determination of indomethacin either in pure form or in capsules has been developed. The method is based on the diazo coupling reaction between indomethacin and a highly reactive arenediazonium ion, 4-carboxyl-2,6-dinitrobenzene diazonium ion, with the consequent formation of an azo dye. The reaction is fast and gave an orange azo dye in ethylacetate. The assay was carried out at 470 nm and the azo adduct was stable for three hours. Beer’s law is obeyed in the concentration range of 3.3-11.55 µg/ml of indomethacin. Optimization studies established an optimum reaction time of 20 minutes at 30o C and the drug –to- reagent ratio of 1:2 for optimal detector response. The method developed has a low limit of detection of 0.90 µgmL-1 and is precise (RSD of 2.3%). The new method has been successfully applied to the determination of indomethacin in capsules and the method is of equivalent accuracy (p> 0.05) with the official (BP) spectrophotometric method. The new method could find application as a simple analytical method for the assay of indomethacin in capsules.

Ključne riječi
indomethacin assay; colorimetry; 4-carboxyl-2,6-dinitrobenzene diazonium; diazo coupling reaction

Hrčak ID: 4555

URI
https://hrcak.srce.hr/4555

[hrvatski]

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