hrcak mascot   Srce   HID

Izvorni znanstveni članak

Separation of Racemic Mixtures of Amino Acids Using Chiral Eluents

P. Dimitrova ; TU Kaiserslautern, Thermische Verfahrenstechnik, P.O. Box 3049; 67653 Kaiserslautern, Germany
H.-J. Bart ; TU Kaiserslautern, Thermische Verfahrenstechnik, P.O. Box 3049; 67653 Kaiserslautern, Germany

Puni tekst: engleski, pdf (226 KB) str. 75-83 preuzimanja: 1.115* citiraj
APA 6th Edition
Dimitrova, P. i Bart, H.-. (2010). Separation of Racemic Mixtures of Amino Acids Using Chiral Eluents. Chemical and Biochemical Engineering Quarterly, 24 (1), 75-83. Preuzeto s https://hrcak.srce.hr/49484
MLA 8th Edition
Dimitrova, P. i H.-J. Bart. "Separation of Racemic Mixtures of Amino Acids Using Chiral Eluents." Chemical and Biochemical Engineering Quarterly, vol. 24, br. 1, 2010, str. 75-83. https://hrcak.srce.hr/49484. Citirano 25.08.2019.
Chicago 17th Edition
Dimitrova, P. i H.-J. Bart. "Separation of Racemic Mixtures of Amino Acids Using Chiral Eluents." Chemical and Biochemical Engineering Quarterly 24, br. 1 (2010): 75-83. https://hrcak.srce.hr/49484
Harvard
Dimitrova, P., i Bart, H.-. (2010). 'Separation of Racemic Mixtures of Amino Acids Using Chiral Eluents', Chemical and Biochemical Engineering Quarterly, 24(1), str. 75-83. Preuzeto s: https://hrcak.srce.hr/49484 (Datum pristupa: 25.08.2019.)
Vancouver
Dimitrova P, Bart H-. Separation of Racemic Mixtures of Amino Acids Using Chiral Eluents. Chemical and Biochemical Engineering Quarterly [Internet]. 2010 [pristupljeno 25.08.2019.];24(1):75-83. Dostupno na: https://hrcak.srce.hr/49484
IEEE
P. Dimitrova i H.-. Bart, "Separation of Racemic Mixtures of Amino Acids Using Chiral Eluents", Chemical and Biochemical Engineering Quarterly, vol.24, br. 1, str. 75-83, 2010. [Online]. Dostupno na: https://hrcak.srce.hr/49484. [Citirano: 25.08.2019.]

Sažetak
A commercially available non-chiral RP-C8 chromatographic column is used with an aqueous chiral mobile phase containing N,N-dimethyl-L-phenylalanine and copper in a ratio 20 : 1 for the enantiomeric resolution of the following amino acids: DL-valine (Val), DL-methionine (Met), DL-leucine (Leu), DL-phenylalanine (Phe) and DL-tyrosine (Tyr). The influence of organic modifier, temperature and flow-rate on the capacity factors and on the selectivity is experimentally determined and the separation behavior is
discussed. The capacity factors of some of the amino acids remained constant with changing the composition of the mobile phase, i.e. increasing methanol amount. With increase of the temperature the retention times decreased and the selectivities were slightly
reduced. In a study of the effect on retention and selectivity, linear van’t Hoff plots were obtained giving ∆H and ∆S values of the solute transfer from the mobile to the stationary phase. These were found to be within the range obtained for hydrophobic compounds in RP-HPLC systems. Using van Deemter plots high separation efficiency and low apparent axial dispersion coefficients for all amino acids could be determined.

Ključne riječi
Amino acid; chiral mobile phase; ligand exchange chromatography; van Deemter plot; van’t Hoff plot

Hrčak ID: 49484

URI
https://hrcak.srce.hr/49484

Posjeta: 1.308 *