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Analysis of Ikaros Family Splicing Variants in Human Hematopoietic Lineages

Maja Matulić ; Department of Molecular Biology, Faculty of Science, Zagreb, Croatia
Mladen Paradžik ; Division of Molecular Biology, Institute »Rudjer Bošković«, Zagreb, Croatia
Biljana Jelić Puskarić ; University Hospital »Merkur«, Zagreb, Croatia
Jagoda Stipić ; University Hospital Center Zagreb, Zagreb, Croatia
Mariastefania Antica ; Division of Molecular Biology, Institute »Rudjer Bošković«, Zagreb, Croatia

Puni tekst: engleski, pdf (108 KB) str. 59-62 preuzimanja: 494* citiraj
APA 6th Edition
Matulić, M., Paradžik, M., Jelić Puskarić, B., Stipić, J. i Antica, M. (2010). Analysis of Ikaros Family Splicing Variants in Human Hematopoietic Lineages. Collegium antropologicum, 34 (1), 59-62. Preuzeto s https://hrcak.srce.hr/51242
MLA 8th Edition
Matulić, Maja, et al. "Analysis of Ikaros Family Splicing Variants in Human Hematopoietic Lineages." Collegium antropologicum, vol. 34, br. 1, 2010, str. 59-62. https://hrcak.srce.hr/51242. Citirano 18.01.2020.
Chicago 17th Edition
Matulić, Maja, Mladen Paradžik, Biljana Jelić Puskarić, Jagoda Stipić i Mariastefania Antica. "Analysis of Ikaros Family Splicing Variants in Human Hematopoietic Lineages." Collegium antropologicum 34, br. 1 (2010): 59-62. https://hrcak.srce.hr/51242
Harvard
Matulić, M., et al. (2010). 'Analysis of Ikaros Family Splicing Variants in Human Hematopoietic Lineages', Collegium antropologicum, 34(1), str. 59-62. Preuzeto s: https://hrcak.srce.hr/51242 (Datum pristupa: 18.01.2020.)
Vancouver
Matulić M, Paradžik M, Jelić Puskarić B, Stipić J, Antica M. Analysis of Ikaros Family Splicing Variants in Human Hematopoietic Lineages. Collegium antropologicum [Internet]. 2010 [pristupljeno 18.01.2020.];34(1):59-62. Dostupno na: https://hrcak.srce.hr/51242
IEEE
M. Matulić, M. Paradžik, B. Jelić Puskarić, J. Stipić i M. Antica, "Analysis of Ikaros Family Splicing Variants in Human Hematopoietic Lineages", Collegium antropologicum, vol.34, br. 1, str. 59-62, 2010. [Online]. Dostupno na: https://hrcak.srce.hr/51242. [Citirano: 18.01.2020.]

Sažetak
Transcription factors from the Ikaros family are involved in lymphocyte differentiation and have a critical role at specific check points of the haemopoietic pathway. However, how developmentally regulated changes are reflected in gene expression programs of lymphocyte differentiation is not well understood. It has been suggested that disregulation of transcription factors from the Ikaros family is associated with the development of different human leukemias. In this work we analyzed the state of Ikaros family members in different leukemic cells with the aim to explore the transcriptional control of human hematopoietic lineages and shed some new light on our understanding of transcription factor significance in human leukemias. By means of RT-PCR and specific primers we investigated the expression of Ikaros, Aiolos and Helios transcription factors and their splicing variants in seven leukemia cell lines derived from different types of leukemia (ALL, CML, AML) and lymphoma (histiocytic lymphoma, Burkitt lymphoma and anaplastic large cell lymphoma). In all of the cell lines examined Ikaros was present in dominant Ik1 to Ik4 isoforms and small Ik6 isoform was absent. Aiolos was expressed in the majority of the cell lines, of both, B and T origin, in the form of the full length Aio1. Helios was also present only in two long isoforms Hel1 and Hel2, and was absent in one third of the lines. Similar distribution of positive and negative expression of Aiolos and Helios found in various types of leukemias could implicate common pathways of their regulation.

Ključne riječi
Ikaros; Aiolos; Helios; transcription factors; gene expression; leukemia

Hrčak ID: 51242

URI
https://hrcak.srce.hr/51242

Posjeta: 760 *