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Izvorni znanstveni članak
Efficiently Activated Serine Analogue is Not Transferred to Yeast tRNASer
Ita Gruić-Sovulj
; Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, 10000 Zagreb, Croatia
Morana Dulić
; Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, 10000 Zagreb, Croatia
Jelena Jarić
; Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, 10000 Zagreb, Croatia
Nevena Cvetešić
; Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, 10000 Zagreb, Croatia
Kristina Majsec
; Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, 10000 Zagreb, Croatia
Ivana Weygand-Đurašević
; Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, 10000 Zagreb, Croatia
| Puni tekst: engleski, pdf (1 MB) | str. 163-169 | citiraj | |
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APA 6th Edition Gruić-Sovulj, I., Dulić, M., Jarić, J., Cvetešić, N., Majsec, K. i Weygand-Đurašević, I. (2010). Efficiently Activated Serine Analogue is Not Transferred to Yeast tRNASer. Croatica Chemica Acta, 83 (2), 163-169. Preuzeto s https://hrcak.srce.hr/56019 MLA 8th Edition Gruić-Sovulj, Ita, et al. "Efficiently Activated Serine Analogue is Not Transferred to Yeast tRNASer." Croatica Chemica Acta, vol. 83, br. 2, 2010, str. 163-169. https://hrcak.srce.hr/56019. Citirano 17.02.2020. Chicago 17th Edition Gruić-Sovulj, Ita, Morana Dulić, Jelena Jarić, Nevena Cvetešić, Kristina Majsec i Ivana Weygand-Đurašević. "Efficiently Activated Serine Analogue is Not Transferred to Yeast tRNASer." Croatica Chemica Acta 83, br. 2 (2010): 163-169. https://hrcak.srce.hr/56019 Harvard Gruić-Sovulj, I., et al. (2010). 'Efficiently Activated Serine Analogue is Not Transferred to Yeast tRNASer', Croatica Chemica Acta, 83(2), str. 163-169. Preuzeto s: https://hrcak.srce.hr/56019 (Datum pristupa: 17.02.2020.) Vancouver Gruić-Sovulj I, Dulić M, Jarić J, Cvetešić N, Majsec K, Weygand-Đurašević I. Efficiently Activated Serine Analogue is Not Transferred to Yeast tRNASer. Croatica Chemica Acta [Internet]. 2010 [pristupljeno 17.02.2020.];83(2):163-169. Dostupno na: https://hrcak.srce.hr/56019 IEEE I. Gruić-Sovulj, M. Dulić, J. Jarić, N. Cvetešić, K. Majsec i I. Weygand-Đurašević, "Efficiently Activated Serine Analogue is Not Transferred to Yeast tRNASer", Croatica Chemica Acta, vol.83, br. 2, str. 163-169, 2010. [Online]. Dostupno na: https://hrcak.srce.hr/56019. [Citirano: 17.02.2020.] |
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Sažetak
Covalent attachment of cognate amino acid to the cognate tRNA is a prerequisite for the faithful synthesis of proteins in the cell. Aminoacylation of tRNA, catalyzed by aminoacyl-tRNA synthetases
(aaRSs), proceeds by a two-step reaction whereby amino acid is first activated and then transferred to the 3'-ribose of tRNA. Serine hydroxamate (SerHX) is an interesting analogue of serine as it exhibits antimicrobial activity due to its inhibition of serylation in yeast and Escherichia coli. SerHX also mimics a noncognate substrate of yeast seryl-tRNA synthetase (ScSerRS) since it is efficiently activated and edited by this enzyme. However, whether this analogue is also transferred to tRNA during the second step of aminoacylation was not previously known. Here we show, for the first time, that aminoacylation of yeast tRNA with SerHX does not occur at a measurable rate, suggesting that the transfer is less tolerable toward SerHX than the activation step.
Ključne riječi
serine hydroxamate; seryl-tRNA synthetase; tRNA; transfer step; amino acid activation
Hrčak ID: 56019
URI
https://hrcak.srce.hr/56019
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