Novel Kinetic Spectrophotometric Method for Determination of Tiopronin [N-(2-Mercaptopropionyl)-glycine]
Lea Kukoc-Modun
orcid.org/0000-0003-1798-6796
; Faculty of Chemistry and Technology, University of Split, Teslina 10/V, 21000 Split, Croatia
Njegomir Radić
; Faculty of Chemistry and Technology, University of Split, Teslina 10/V, 21000 Split, Croatia
APA 6th Edition Kukoc-Modun, L. i Radić, Nj. (2010). Novel Kinetic Spectrophotometric Method for Determination of Tiopronin [N-(2-Mercaptopropionyl)-glycine]. Croatica Chemica Acta, 83 (2), 189-195. Preuzeto s https://hrcak.srce.hr/56022
MLA 8th Edition Kukoc-Modun, Lea i Njegomir Radić. "Novel Kinetic Spectrophotometric Method for Determination of Tiopronin [N-(2-Mercaptopropionyl)-glycine]." Croatica Chemica Acta, vol. 83, br. 2, 2010, str. 189-195. https://hrcak.srce.hr/56022. Citirano 26.01.2021.
Chicago 17th Edition Kukoc-Modun, Lea i Njegomir Radić. "Novel Kinetic Spectrophotometric Method for Determination of Tiopronin [N-(2-Mercaptopropionyl)-glycine]." Croatica Chemica Acta 83, br. 2 (2010): 189-195. https://hrcak.srce.hr/56022
Harvard Kukoc-Modun, L., i Radić, Nj. (2010). 'Novel Kinetic Spectrophotometric Method for Determination of Tiopronin [N-(2-Mercaptopropionyl)-glycine]', Croatica Chemica Acta, 83(2), str. 189-195. Preuzeto s: https://hrcak.srce.hr/56022 (Datum pristupa: 26.01.2021.)
Vancouver Kukoc-Modun L, Radić Nj. Novel Kinetic Spectrophotometric Method for Determination of Tiopronin [N-(2-Mercaptopropionyl)-glycine]. Croatica Chemica Acta [Internet]. 2010 [pristupljeno 26.01.2021.];83(2):189-195. Dostupno na: https://hrcak.srce.hr/56022
IEEE L. Kukoc-Modun i Nj. Radić, "Novel Kinetic Spectrophotometric Method for Determination of Tiopronin [N-(2-Mercaptopropionyl)-glycine]", Croatica Chemica Acta, vol.83, br. 2, str. 189-195, 2010. [Online]. Dostupno na: https://hrcak.srce.hr/56022. [Citirano: 26.01.2021.]
Sažetak Novel simple kinetic spectrophotometric method for the determination of tiopronin [N-(2-mercaptopropionyl)-glycine, MPG] in pharmaceutical formulation has been developed and validated. The
proposed method is based on the coupled redox-complexation reaction whose first step is reduction of
FeIII by MPG, while second one includes the complexation of FeII, resulted from preceding redox reaction,
with 2,4,6-trypyridyl-s-triazine (TPTZ). The stable Fe(TPTZ)22+ complex exhibits an absorption maximum
at = 593 nm. The initial rate and fixed time (at 3 min) methods were utilized for constructing the calibration graphs.
The graphs were linear in concentration ranges from 1.0 10−6 to 1.0 10−4 mol L−1 for both methods with
limits of detection 1.3 10−7 mol L−1 and 7.5 10−8 mol L−1 for the initial rate and fixed time method, respectively.
The proposed methods were successfully applied for the determination of MPG in its commercial
pharmaceutical formulation.