Veterinarski arhiv, Vol. 80 No. 2, 2010.
Izvorni znanstveni članak
Prevalence of aerobactin and adhesin genes in Escherichia coli isolates from blood of bacteremic severely ill neonatal calves
Reza Ghanbarpour
; Department of Microbiology, Molecular Microbiology Group, Faculty of Veterinary Medicine, Shahid Bahonar University, Kerman, Iran
Mohammad N. Nazem
; Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University, Kerman, Iran
Sažetak
Escherichia coli isolates are one of the most prevalent bacteria in the blood of bacteremic calves. Twenty two E. coli were isolated from the blood of severely diseased calves. The O-serogroup of E. coli isolates was determined. The presence and prevalence of virulence genes included iucD for (aerobactin), afaE-VIII (afimbrial adhesin), clpG (CS31A adhesin), f5 (F5 fimbria), f17A (F17 fimbrial family), f17a-A, f17b-A, f17c-A and f17d-A genes (F17a-A. F17b-A, F17c-A and F17d-A fimbria), f41 (F41 fi mbria) and papC (P fimbrial adhesin) were examined by PCR assays. Nine different O serogroup O78 (3 isolates), O115 (2), O21 (2), O8 (1), O9 (1), O15 (1), O20 (1), O141 (1) and O153 (1) were determined and 9 isolates were nontypeable. Most of the isolates (20/22) were positive for at least one of the examined genes. All the isolates were negative for F5, F41, P, F17a-A and F17b-A encoding genes. The iucD was the most prevalent virulence gene with positive isolates belonging to 5 serogroups. Eight f17A positive isolates belonged to f17b-A and f17c-A variants equally. CS31A and AfaE-VIII encoding genes were detected in 6 and 4 isolates respectively. Six different combinations of virulence genes were detected: the two most prevalent combinations of afaE-VIII- f17A and iucD-clpG were found in 4 and 3 isolates respectively.
Ključne riječi
Escherichia coli; aerobactin; adhesin; bacteremia; calf
Hrčak ID:
56259
URI
Datum izdavanja:
26.4.2010.
Posjeta: 1.311 *