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Original scientific paper

Insulin Administration in the Mild Hyperglycaemia Changes Expression of Proinflammatory Adhesion Molecules on Human Aortic Endothelial Cells

Tatjana Bačun
Ljubica Glavaš-Obrovac
Tatjana Belovari
Ivan Mihaljević
Toni Hanich
Vesna Feher Belaj
Aleksandar Včev

Fulltext: english, pdf (297 KB) pages 911-915 downloads: 343* cite
APA 6th Edition
Bačun, T., Glavaš-Obrovac, Lj., Belovari, T., Mihaljević, I., Hanich, T., Feher Belaj, V. & Včev, A. (2010). Insulin Administration in the Mild Hyperglycaemia Changes Expression of Proinflammatory Adhesion Molecules on Human Aortic Endothelial Cells. Collegium antropologicum, 34 (3), 911-915. Retrieved from https://hrcak.srce.hr/59332
MLA 8th Edition
Bačun, Tatjana, et al. "Insulin Administration in the Mild Hyperglycaemia Changes Expression of Proinflammatory Adhesion Molecules on Human Aortic Endothelial Cells." Collegium antropologicum, vol. 34, no. 3, 2010, pp. 911-915. https://hrcak.srce.hr/59332. Accessed 28 Nov. 2021.
Chicago 17th Edition
Bačun, Tatjana, Ljubica Glavaš-Obrovac, Tatjana Belovari, Ivan Mihaljević, Toni Hanich, Vesna Feher Belaj and Aleksandar Včev. "Insulin Administration in the Mild Hyperglycaemia Changes Expression of Proinflammatory Adhesion Molecules on Human Aortic Endothelial Cells." Collegium antropologicum 34, no. 3 (2010): 911-915. https://hrcak.srce.hr/59332
Harvard
Bačun, T., et al. (2010). 'Insulin Administration in the Mild Hyperglycaemia Changes Expression of Proinflammatory Adhesion Molecules on Human Aortic Endothelial Cells', Collegium antropologicum, 34(3), pp. 911-915. Available at: https://hrcak.srce.hr/59332 (Accessed 28 November 2021)
Vancouver
Bačun T, Glavaš-Obrovac Lj, Belovari T, Mihaljević I, Hanich T, Feher Belaj V, et al. Insulin Administration in the Mild Hyperglycaemia Changes Expression of Proinflammatory Adhesion Molecules on Human Aortic Endothelial Cells. Collegium antropologicum [Internet]. 2010 [cited 2021 November 28];34(3):911-915. Available from: https://hrcak.srce.hr/59332
IEEE
T. Bačun, et al., "Insulin Administration in the Mild Hyperglycaemia Changes Expression of Proinflammatory Adhesion Molecules on Human Aortic Endothelial Cells", Collegium antropologicum, vol.34, no. 3, pp. 911-915, 2010. [Online]. Available: https://hrcak.srce.hr/59332. [Accessed: 28 November 2021]

Abstracts
An overexpression of cell adhesion molecules (CAMs) on the surface of endothelial cells is one of the first steps in a high glucose-mediated endothelial dysfunction in diabetic patients. The effect of insulin administration in the condition of elevated glucose concentration on the E-selectin, intracellular adhesion molecule-1 (ICAM-1) and vascular adhesion molecule-1 (VCAM-1) expression on human aortic endothelial cells (HAEC) was investigated. Cells were cultured for 4h in a medium supplemented with homocysteine (7 mM) and different concentration of glucose (5.5, 8.0, 12.0 and 16.5 mM respectively) with or without insulin (1 mIU/mL) addition. Expression of CAMs was analysed by flow-cytometry using monoclonal antibodies. Controls were CAMs expression in the medium with a corresponding glucose concentration. Obtained results show that short-term exposure of HAECs to moderate high glucose concentrations results in increased expression of E-selectin (2-fold), VCAM-1 (3-fold) and ICAM-1 (47%). At the same time, HAEC grown with 12 mM glucose expressed lesser E-selectin and, more ICAM-1 (for 64%) and VCAM-1 (41%) molecules. 16.5 mM glucose decreased expression of all investigated adhesion molecules. Addition of insulin was not changed expression of CAMs in a medium with 5.5 mM glucose. In conditions of elevated glucose concentration (12 mM), addition of insulin significantly dropped E-selectin (27%) and increased VCAM-1 (23%) expression. In conclusion, moderate elevated glucose concentration increased expression of cell adhesion molecules on HAEC. Insulin administration in the mild hyperglycaemia reduces an expression of the proinflammatory adhesion molecule E-selectin which could contribute in deceleration of macrovascular complications development in diabetic patients.

Keywords
cell adhesion molecules; mild hyperglycaemia; insulin; human aortic endothelial cells

Hrčak ID: 59332

URI
https://hrcak.srce.hr/59332

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