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Interaction of High Density Lipoprotein with Nicotine – an IR and Raman Study

Dubravka Krilov ; Department of Physics and Biophysics, School of Medicine, University of Zagreb, Šalata 3b, Zagreb, HR-10000 Croatia
Marin Kosović ; Department of Physics and Biophysics, School of Medicine, University of Zagreb, Šalata 3b, Zagreb, HR-10000 Croatia
Maja Balarin ; Department of Physics and Biophysics, School of Medicine, University of Zagreb, Šalata 3b, Zagreb, HR-10000 Croatia
Ozren Gamulin ; Department of Physics and Biophysics, School of Medicine, University of Zagreb, Šalata 3b, Zagreb, HR-10000 Croatia
Jasminka Brnjas-Kraljević ; Department of Physics and Biophysics, School of Medicine, University of Zagreb, Šalata 3b, Zagreb, HR-10000 Croatia

Puni tekst: engleski, pdf (903 KB) str. 387-393 preuzimanja: 973* citiraj
APA 6th Edition
Krilov, D., Kosović, M., Balarin, M., Gamulin, O. i Brnjas-Kraljević, J. (2010). Interaction of High Density Lipoprotein with Nicotine – an IR and Raman Study. Croatica Chemica Acta, 83 (4), 387-393. Preuzeto s https://hrcak.srce.hr/62641
MLA 8th Edition
Krilov, Dubravka, et al. "Interaction of High Density Lipoprotein with Nicotine – an IR and Raman Study." Croatica Chemica Acta, vol. 83, br. 4, 2010, str. 387-393. https://hrcak.srce.hr/62641. Citirano 27.11.2021.
Chicago 17th Edition
Krilov, Dubravka, Marin Kosović, Maja Balarin, Ozren Gamulin i Jasminka Brnjas-Kraljević. "Interaction of High Density Lipoprotein with Nicotine – an IR and Raman Study." Croatica Chemica Acta 83, br. 4 (2010): 387-393. https://hrcak.srce.hr/62641
Harvard
Krilov, D., et al. (2010). 'Interaction of High Density Lipoprotein with Nicotine – an IR and Raman Study', Croatica Chemica Acta, 83(4), str. 387-393. Preuzeto s: https://hrcak.srce.hr/62641 (Datum pristupa: 27.11.2021.)
Vancouver
Krilov D, Kosović M, Balarin M, Gamulin O, Brnjas-Kraljević J. Interaction of High Density Lipoprotein with Nicotine – an IR and Raman Study. Croatica Chemica Acta [Internet]. 2010 [pristupljeno 27.11.2021.];83(4):387-393. Dostupno na: https://hrcak.srce.hr/62641
IEEE
D. Krilov, M. Kosović, M. Balarin, O. Gamulin i J. Brnjas-Kraljević, "Interaction of High Density Lipoprotein with Nicotine – an IR and Raman Study", Croatica Chemica Acta, vol.83, br. 4, str. 387-393, 2010. [Online]. Dostupno na: https://hrcak.srce.hr/62641. [Citirano: 27.11.2021.]

Sažetak
The direct interaction of high density lipoprotein (HDL) with nicotine, one of the major components
of cigarette particulate matter, has been studied here at molecular level. Nicotine affects the vibration
modes of HDL due to its embedment within the lipid monolayer. The changes in the positions and intensities
of vibration bands in protein and lipid domain of the particle were studied by IR and Raman
spectroscopy. Three types of samples were prepared: native HDL sample and two HDL samples with
added nicotine. The molecular ratio of nicotine /phospholipids in two samples was 1: 12 and 1: 6. The
same types of samples were prepared from liposomes containing phosphatidylcholine, and sphingomyelin
with addition of cholesterol. Spectra of liposome samples were used for distinguishing and attribution of
lipid bands in spectra of HDL samples, where the majority of changes were observed. The incorporation
of nicotine into lipid monolayer induces changes in the lipid bands from the vibrations in acyl chains and
head groups of phospholipids. The changes in vibration bands from particular amino acids' residues confirm
that nicotine molecule is located within lipid monolayer but close to lipid-protein interface.

Ključne riječi
HDL; nicotine; liposome; IR spectroscopy; Raman spectroscopy

Hrčak ID: 62641

URI
https://hrcak.srce.hr/62641

Posjeta: 1.360 *