Izvorni znanstveni članak
Relationship Between Vascular Endothelial Growth Factor and Nuclear Factor-κB in Renal Cell Tumors
Gordana Đorđević
orcid.org/0000-0002-2010-6203
; Department of Pathology, Rijeka University School of Medicine, Rijeka, Croatia
Koviljka Matušan-Ilijaš
; Department of Pathology, Rijeka University School of Medicine, Rijeka, Croatia
Emina Sinožić
; Department of Pathology, Rijeka University School of Medicine, Rijeka, Croatia
Giuseppe Damante
; Dipartimento di Scienze e Tecnologie Biomediche, Universita degli Studi di Udine, Italy
Dora Fabbro
; Azienda Ospedaliero Universitaria S. Maria della Misericordia, Udine, Italy
Blaženka Grahovac
; Department of Pathology, Rijeka University School of Medicine, Rijeka, Croatia
Ksenija Lučin
; Department of Pathology, Rijeka University School of Medicine, Rijeka, Croatia
Nives Jonjić
orcid.org/0000-0003-2995-0766
; Department of Pathology, Rijeka University School of Medicine, Rijeka, Croatia
Sažetak
Aim To assess the relationship between protein and messenger RNA
(mRNA) levels of vascular endothelial growth factor (VEGF) and subcellular
localization of nuclear factor-kappa B (NF-κB), proliferation rate of
tumor cells, and clinicopathological characteristics of renal cell tumors.
Methods We analyzed 31 one renal cell tumors – 22 clear cell renal cell
carcinomas (CCRCC) and 9 other histologic types (non-CCRCC).
VEGF expression and subcellular localization of p65 member of NF-κB
and Ki67 were immunohistochemically evaluated for the proliferation
rate of tumor cells. Expression of VEGF mRNA was assessed using quantitative
real-time polymerase chain reaction after total RNA extraction
from snap-frozen tumor tissue samples.
Results Cytoplasmic localization of VEGF protein in renal cell tumors
showed a perimembranous and diffuse pattern, the former being more
evident in CCRCC (27.1 ± 18.9 vs 3.3 ± 10 % tumors, P<0.001) and
the latter in non-CCRCC type (71.7 ± 23.2 vs 31.1 ± 22.1 % tumors,
P < 0.001). Heterogeneity in VEGF gene expression was more pronounced
in CCRCC type than in non-CCRCC type (P = 0.004). In addition,
perimembranous VEGF pattern was associated with higher VEGF
mRNA levels (P = 0.006) and diffuse VEGF pattern with lower VEGF
mRNA levels (P < 0.001). Nuclear and cytoplasmic staining of NF-κB/
p65 was observed in the majority of tumor cells. A significant association
was recorded between cytoplasmic NK-κB/65 staining and VEGF staining
of diffuse pattern (P = 0.026). Association between NF-κB/65 and
proliferation rate of tumor cells was significant for cytoplasmic staining
(P = 0.039) but not for nuclear NFkB/p65 staining (P = 0.099).
Conclusion Higher but inhomogeneous expression of VEGF in tumor
cells, especially in CCRCCs, is associated with NF-κB/65 activity. This
indicates that both VEGF and NF-κB/65 may be important in renal carcinogenesis,
representing a possible molecular target in the treatment of
renal cell carcinoma.
Ključne riječi
Carcinoma; Renal Cell; Nuclear Factor kappa B; Immunohistochemistry; Polymerase Chain Reaction; VEGF
Hrčak ID:
29274
URI
Datum izdavanja:
15.10.2008.
Posjeta: 1.446 *