hrcak mascot   Srce   HID

Croatian medical journal, Vol. 49 No. 5, 2008.

Izvorni znanstveni članak

Relationship Between Vascular Endothelial Growth Factor and Nuclear Factor-κB in Renal Cell Tumors

Gordana Đorđević   ORCID icon orcid.org/0000-0002-2010-6203 ; Department of Pathology, Rijeka University School of Medicine, Rijeka, Croatia
Koviljka Matušan-Ilijaš ; Department of Pathology, Rijeka University School of Medicine, Rijeka, Croatia
Emina Sinožić ; Department of Pathology, Rijeka University School of Medicine, Rijeka, Croatia
Giuseppe Damante ; Dipartimento di Scienze e Tecnologie Biomediche, Universita degli Studi di Udine, Italy
Dora Fabbro ; Azienda Ospedaliero Universitaria S. Maria della Misericordia, Udine, Italy
Blaženka Grahovac ; Department of Pathology, Rijeka University School of Medicine, Rijeka, Croatia
Ksenija Lučin ; Department of Pathology, Rijeka University School of Medicine, Rijeka, Croatia
Nives Jonjić   ORCID icon orcid.org/0000-0003-2995-0766 ; Department of Pathology, Rijeka University School of Medicine, Rijeka, Croatia

Puni tekst: engleski, pdf (694 KB) str. 608-617 preuzimanja: 221* citiraj
APA 6th Edition
Đorđević, G., Matušan-Ilijaš, K., Sinožić, E., Damante, G., Fabbro, D., Grahovac, B., ... Jonjić, N. (2008). Relationship Between Vascular Endothelial Growth Factor and Nuclear Factor-κB in Renal Cell Tumors. Croatian medical journal, 49 (5), 608-617. Preuzeto s https://hrcak.srce.hr/29274
MLA 8th Edition
Đorđević, Gordana, et al. "Relationship Between Vascular Endothelial Growth Factor and Nuclear Factor-κB in Renal Cell Tumors." Croatian medical journal, vol. 49, br. 5, 2008, str. 608-617. https://hrcak.srce.hr/29274. Citirano 25.04.2019.
Chicago 17th Edition
Đorđević, Gordana, Koviljka Matušan-Ilijaš, Emina Sinožić, Giuseppe Damante, Dora Fabbro, Blaženka Grahovac, Ksenija Lučin i Nives Jonjić. "Relationship Between Vascular Endothelial Growth Factor and Nuclear Factor-κB in Renal Cell Tumors." Croatian medical journal 49, br. 5 (2008): 608-617. https://hrcak.srce.hr/29274
Harvard
Đorđević, G., et al. (2008). 'Relationship Between Vascular Endothelial Growth Factor and Nuclear Factor-κB in Renal Cell Tumors', Croatian medical journal, 49(5), str. 608-617. Preuzeto s: https://hrcak.srce.hr/29274 (Datum pristupa: 25.04.2019.)
Vancouver
Đorđević G, Matušan-Ilijaš K, Sinožić E, Damante G, Fabbro D, Grahovac B i sur. Relationship Between Vascular Endothelial Growth Factor and Nuclear Factor-κB in Renal Cell Tumors. Croat Med J. [Internet]. 2008 [pristupljeno 25.04.2019.];49(5):608-617. Dostupno na: https://hrcak.srce.hr/29274
IEEE
G. Đorđević, et al., "Relationship Between Vascular Endothelial Growth Factor and Nuclear Factor-κB in Renal Cell Tumors", Croatian medical journal, vol.49, br. 5, str. 608-617, 2008. [Online]. Dostupno na: https://hrcak.srce.hr/29274. [Citirano: 25.04.2019.]

Sažetak
Aim To assess the relationship between protein and messenger RNA
(mRNA) levels of vascular endothelial growth factor (VEGF) and subcellular
localization of nuclear factor-kappa B (NF-κB), proliferation rate of
tumor cells, and clinicopathological characteristics of renal cell tumors.
Methods We analyzed 31 one renal cell tumors – 22 clear cell renal cell
carcinomas (CCRCC) and 9 other histologic types (non-CCRCC).
VEGF expression and subcellular localization of p65 member of NF-κB
and Ki67 were immunohistochemically evaluated for the proliferation
rate of tumor cells. Expression of VEGF mRNA was assessed using quantitative
real-time polymerase chain reaction after total RNA extraction
from snap-frozen tumor tissue samples.
Results Cytoplasmic localization of VEGF protein in renal cell tumors
showed a perimembranous and diffuse pattern, the former being more
evident in CCRCC (27.1 ± 18.9 vs 3.3 ± 10 % tumors, P<0.001) and
the latter in non-CCRCC type (71.7 ± 23.2 vs 31.1 ± 22.1 % tumors,
P < 0.001). Heterogeneity in VEGF gene expression was more pronounced
in CCRCC type than in non-CCRCC type (P = 0.004). In addition,
perimembranous VEGF pattern was associated with higher VEGF
mRNA levels (P = 0.006) and diffuse VEGF pattern with lower VEGF
mRNA levels (P < 0.001). Nuclear and cytoplasmic staining of NF-κB/
p65 was observed in the majority of tumor cells. A significant association
was recorded between cytoplasmic NK-κB/65 staining and VEGF staining
of diffuse pattern (P = 0.026). Association between NF-κB/65 and
proliferation rate of tumor cells was significant for cytoplasmic staining
(P = 0.039) but not for nuclear NFkB/p65 staining (P = 0.099).
Conclusion Higher but inhomogeneous expression of VEGF in tumor
cells, especially in CCRCCs, is associated with NF-κB/65 activity. This
indicates that both VEGF and NF-κB/65 may be important in renal carcinogenesis,
representing a possible molecular target in the treatment of
renal cell carcinoma.

Ključne riječi
Carcinoma; Renal Cell; Nuclear Factor kappa B; Immunohistochemistry; Polymerase Chain Reaction; VEGF

Hrčak ID: 29274

URI
https://hrcak.srce.hr/29274

Posjeta: 391 *