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EPIDEMIOLOGICAL INVESTIGATION OF ANIMAL DISEASES CAUSED BY CLOSTRIDIUM PERFRINGENS STRAINS ISOLATED FROM FEEDINGSTUFFS
Elżbieta Kukier
; National Veterinary Research Institute, Pulawy, Poland
Magdalena Goldsztejn
; National Veterinary Research Institute, Pulawy, Poland
Krzysztof Kwiatek
; National Veterinary Research Institute, Pulawy, Poland
Sažetak
C. perfringens is the primary cause of clostridial enteric diseases in domestic animals. Diagnostic difficulties result from common occurrence of these microorganisms in the intestinal tract of healthy vertebrates and laboratory serotyping methods cannot discriminate isolates reliably. C. perfringens caused disease may be confirmed by presence of these bacteria in feed and a, high number of these anaerobes in animal intestinal tract by typing methods of comparably high differentiation power. In this study C. perfringens strains isolated from compound feedingstuffs and poultry faeces were enumerated and characterized genotypically for toxin genes presence by mPCR. Presence of C. perfringens at the level higher than 10 cfu/g was confirmed in 68% in feed isolates and in 36% in poultry isolates. Dominance of C. perfringens strains type A was confirmed among isolated strains from feeds and poultry. Nearly 42% of isolates from feeds and 4% from poultry belonged to subtype β2. There were some positive isolates for netB gene. The obtained results of analyses revealed common occurrence status of C. perfringens in feeds and clinically healthy birds
Ključne riječi
Clostridium perfringens; feedingstuffs; poultry; necrotic enteritis
Hrčak ID:
68048
URI
Datum izdavanja:
8.4.2011.
Posjeta: 968 *