Izvorni znanstveni članak

Influence of in vitro preconditioning of citrus microshoots with paclobutrazol on ex vitro survival

B.N. Hazarika ; ICAR Research Complex for Neh Region, Meghalaya, India
V.A. Parthasarathy ; Division of Crop Improvement, Central Plantation Crop Research Institute, Kerala, India
V. Nagaraju ; ICAR Research Complex for Neh Region, Meghalaya, India

Sažetak

The influence of the in vitro conditioning and rooting of the microcuttings in different basal media viz., Murashige and Skoog and Gamborg supplemented with various concentrations of paclobutrazol (0.25-4.0 mg/1) on their in vivo establishment and growth was investigated using four citrus species viz., C. reticulata Blanco (Khasi Mandarin), C. volkameriana Ten, and Pasq, C. reshni Tanaka (Cleopatra Mandarin), and C. nobilis X C. deliciosa Tenure (Kinnow). The inclusion of paclobutrazol in the medium had a significant influence on in vivo growth especially in relation to height. Paclobutrazol reduced the stem and root growth but at 0.75 and 1.0 mg/1 more persistent leaves and new leaves on established plants were recorded. Stomata of leaves cultured in medium supplemented with paclobutrazol showed smaller stomatal apertures. Among the species, Kinnow and C. volkameriana showed the maximum leaf area. The optimum concentration of paclobutrazol for maximum establishment was found to be 1 mg/1 paclobutrazol (94.6% to 97.2 %) in all the species.

Ključne riječi

citrus; culture; paclobutrazol; survival; growth; root; stem; leaves

Hrčak ID:

160811

URI

https://hrcak.srce.hr/160811

Datum izdavanja:

31.12.2001.

Posjeta: 712 *