Sažetak sa skupa
Regenerative Dentistry - A Preliminary Study on Tooth Germs of the Mouse
F. Nagel
Sažetak
OBJECTIVES: One of the most important bases in regenerative dentistry is the understanding of cell differentiation of the tooth germ. The preliminary study comprises the following: 1. micropreparation of tooth germs of the mouse and 2. cell examination after different periods of defined cell cultivation.
MATERIAL AND METHODS: The tooth germs were gently removed from mice under the microscope by means of micropreparation techniques. All of the germs were embedded in agar, positioned on Millipore Filters and cultivated over 14 and 21 days in a chemically
defined medium. For microscopic examination the germs were fixed, cut and stained with Giemsa- Romanowski and HE.
RESULTS: A cell layer on the Millipore Filters formed, which onginated either from dental pulp or from the outer enamel epithelium. Cells from the cell periphery appeared oblong with broad intermembranous areas. In the cell center the closely closed-up cells exhibited cubic cell form. After 14 days the cell nucleus appeared round and light blue after staining with Giemsa-Romanowski. In contrast, after 21 days a dark stained nucloeplasma was identified in the cells.
CONCLUSION: The results agree with previous studies on which tooth germs can be cultivated successfully in vitro. After the first observations the tooth germs did not show temporally co-ordinated growth and no differentiation as under in vivo conditions. A substantial reason for this lies in the lack of knowledge of the accurate environmental conditions necessery in vitro. With the cell
layer on the Milliopore Filters described here, the possibility of further investigations of cell differentiation exists.
Ključne riječi
Hrčak ID:
10192
URI
Datum izdavanja:
15.9.2002.
Posjeta: 1.296 *