Izvorni znanstveni članak
Changes in the composition of salivary aminoacids, proteins and enzymes in children with phenylketonuria
Ljubica Vranić-Stavljenić
; Siget Health Center, Croatia
Zdravko Rajić
; Unit of Children’s and Preventive Dentistry, School of Dentistry, University of Zagreb, Zagreb,Croatia
Dubravka Čvorišćec
; Unit of Children’s and Preventive Dentistry, School of Dentistry, University of Zagreb, Zagreb, Croatia
Paula Granić
; Unit of Children’s and Preventive Dentistry, School of Dentistry, University of Zagreb, Zagreb, Croatia
Duško Mardešić
; Institute of Clinical Laboratory Diagnostics and Department of Pediatrics
Marinko Zaninović
; Rebro Clinical Hospital Center, University of Zagreb
Zorislav Devernay
; Hospital of Chronic C hildren’s Diseases, Gornja Bistra
Sažetak
Since children with phenylketonuria have a low incidence of caries, the aim of this study was to examine the changes occuring in the aminoacid and protein composition as well as in the composition of salivary enzymes, which might serve as biochemical indicators, representing the factors of predisposition to the development of caries. For this purpose, the analysis was carried out in the native, unstimulated, mixed salivary specimens taken from the sublingual region of children with late diagnosis of RKU (n — 16), those with early diagnosis of the disorder by means of a systematic diagnostic screeniig program for PKU using Guthrie’s test (n — 7), and a control group of children free of any disturbances of the amino-acid metabolism (n — 44). A method of qualitative chromatography on silica gel was used for separation of aminoacids, while the proportions of each individual aminoacid were determined by a densitometer. The content of total salivary proteins was assessed by the method of Lowry, and the concentration of lysozyme by the method of Prockop. Differences in particular proportions of aminoacids, and an increase in the total protein value as well as in the lysozyme activity, could be taken as the factors of predisposition or resistance to caries.
Ključne riječi
phenylketonuria; saliva; caries
Hrčak ID:
105110
URI
Datum izdavanja:
15.6.1987.
Posjeta: 1.150 *