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https://doi.org/10.2478/10004-1254-65-2014-2421

First evidence of the P-glycoprotein gene expression and multixenobiotic resistance modulation in earthworm

Ivana Bošnjak ; Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, Zagreb, Croatia
Ana Bielen ; Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, Zagreb, Croatia
Sanja Babić ; Division of Materials Chemistry, Ruđer Bošković Institute, Zagreb, Croatia
Lidija Šver ; Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, Zagreb, Croatia
Natalija Topić Popović ; Division of Materials Chemistry, Ruđer Bošković Institute, Zagreb, Croatia
Ivančica Strunjak-Perović ; Division of Materials Chemistry, Ruđer Bošković Institute, Zagreb, Croatia
Rozelinda Čož-Rakovac ; Division of Materials Chemistry, Ruđer Bošković Institute, Zagreb, Croatia
Roberta Sauerborn Klobučar ; Division of Materials Chemistry, Ruđer Bošković Institute, Zagreb, Croatia


Puni tekst: engleski pdf 802 Kb

str. 67-74

preuzimanja: 778

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Sažetak

Multixenobiotic resistance (MXR) is an important mechanism of cellular efflux mediated by ATP binding cassette (ABC) transporters that bind and actively remove toxic substrates from the cell. This study was the first to identify ABC transporter P-glycoprotein (P-gp/ABCB1) as a representative of the MXR phenotype in earthworm (Eisenia fetida). The identified partial cDNA sequence of ABCB1 overlapped with ABCB1 homologues of other organisms from 58.5 % to 72.5 %. We also studied the effect of five modulators (verapamil, cyclosporine A, MK571, probenecid, and orthovanadate) on the earthworm’s MXR activity by measuring the accumulation of model substrates rhodamine B and rhodamine 123 in whole body tissue of the adult earthworm. MK571, orthovanadate, and verapamil significantly inhibited MXR activity, and rhodamine 123 turned out to better reflect MXR activity in that species than rhodamine B. Our results show that E. fetida can serve well as a test organism for environmental pollutants that inhibit MXR activity.

Ključne riječi

ABC transport proteins, cellular detoxification, cyclosporine A, Eisenia fetida, MK571, MXR, orthovanadate, probenecid, soil organism, verapamil

Hrčak ID:

117132

URI

https://hrcak.srce.hr/117132

Podaci na drugim jezicima: hrvatski

Posjeta: 1.477 *