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Arhiv za higijenu rada i toksikologiju, Vol. 65 No. 3, 2014.

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https://doi.org/10.2478/10004-1254-65-2014-2538

Different enzymatic activities in carp (Cyprinus carpio L.) as potential biomarkers of exposure to the pesticide methomyl

David Hernández-Moreno ; Toxicology Unit, Veterinary School of Caceres, Caceres, Spain, Universidad Autónoma de Chile, Chile
Irene de la Casa-Resino ; Toxicology Unit, Veterinary School of Caceres, Caceres, Spain
José María Flores ; Toxicology Unit, Veterinary School of Caceres, Caceres, Spain
Manuel José González-Gómez ; Toxicology Unit, Veterinary School of Caceres, Caceres, Spain
Carlos María Neila ; Toxicology Unit, Veterinary School of Caceres, Caceres, Spain
Francisco Soler ; Toxicology Unit, Veterinary School of Caceres, Caceres, Spain
Marcos Pérez-López ; Toxicology Unit, Veterinary School of Caceres, Caceres, Spain

Puni tekst: engleski pdf 431 Kb

str. 311-317

preuzimanja: 1.860

citiraj

Sažetak

This study investigated the influence of the pesticide methomyl on different enzymatic activities in carp. The fish were exposed to a sub-lethal concentration (0.34 mg L-1) of methomyl for 15 days. On days 4 and 15, catalase (CAT) and glutathione-S-transferase (GST) activities were measured in the liver and gills. Acetylcholinesterase (AChE) activity in brain and muscle was also determined. Liver catalase activity slightly increased in exposed fish when compared to controls, but it was statistically significant only at the beginning of the experiment. No changes in CAT activity in the gills of exposed and control animals were observed (mean values were in the range 10.7-11.7 nmol min-1 per mg of protein). Liver GST activity was slightly inhibited in the exposed animals at the beginning of the study; however, it was significantly inhibited in the gills. Brain AChE activity was diminished throughout the experiment and significantly decreased after 96 h of exposure compared to controls (0.041 vs. 0.075 nmol min-1 per mg of protein; p<0.001). Our findings suggest that CAT, GST, and AChE are reliable biomarkers of effect after exposure to methomyl.

Ključne riječi

acetylcholinesterase; carbamate; catalase; fish; glutathione S-transferase

Hrčak ID:

126614

URI

https://hrcak.srce.hr/126614

Datum izdavanja:

11.9.2014.

Podaci na drugim jezicima: hrvatski

Posjeta: 2.714 *