Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2014.55.115
Improved visibility of character conflicts in quasi-median networks with the EMPOP NETWORK software
Bettina Zimmermann
; Institute of Legal Medicine, Innsbruck Medical University Innsbruck, Austria
Alexander W. Röck
; Institute of Legal Medicine, Innsbruck Medical University Innsbruck, Austria
Arne Dür
; Institute of Mathematics University of Innsbruck, Innsbruck, Austria
Walther Parson
; Institute of Legal Medicine, Innsbruck Medical University Innsbruck, Austria
Sažetak
Aim To provide a valuable tool for graphical representation
of mitochondrial DNA (mtDNA) data that enables visual
emphasis on complex substructures within the network
to highlight possible ambiguities and errors.
Method We applied the new NETWORK graphical user
interface, available via EMPOP (European DNA Profiling
Group Mitochondrial DNA Population Database; www.
empop.org) by means of two mtDNA data sets that were
submitted for quality control.
Results The quasi-median network torsi of the two data
sets resulted in complex reticulations, suggesting ambiguous
data. To check the corresponding raw data, accountable
nodes and connecting branches of the network
could be identified by highlighting induced subgraphs
with concurrent dimming of their complements. This is
achieved by accentuating the relevant substructures in
the network: mouse clicking on a node displays a list of
all mtDNA haplotypes included in that node; the selection
of a branch specifies the mutation(s) connecting
two nodes. It is indicated to evaluate these mutations by
means of the raw data.
Conclusion Inspection of the raw data confirmed the
presence of phantom mutations due to suboptimal electrophoresis
conditions and data misinterpretation. The
network software proved to be a powerful tool to highlight
problematic data and guide quality control of mtDNA
data tables.
Ključne riječi
Hrčak ID:
127276
URI
Datum izdavanja:
15.4.2014.
Posjeta: 988 *