Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2014.55.195
A comparative study of neurotoxic potential of synthesized polysaccharidecoated and native ferritinbased magnetic nanoparticles
Arseniy Borysov
; Palladin Institute of Biochemistry National Academy of Sciences of Ukraine, Kiev, Ukraine
Natalia Krisanova
; Palladin Institute of Biochemistry National Academy of Sciences of Ukraine, Kiev, Ukraine
Olexander Chunihin
; Palladin Institute of Biochemistry National Academy of Sciences of Ukraine, Kiev, Ukraine
Ludmila Ostapchenko
; Educational and Scientific Center “Institute of Biology,” Taras Shevchenko National University in Kiev, Kiev, Ukraine
Nataliya Pozdnyakova
; Palladin Institute of Biochemistry National Academy of Sciences of Ukraine, Kiev, Ukraine
Тatiana Borisova
; Palladin Institute of Biochemistry National Academy of Sciences of Ukraine, Kiev, Ukraine
Sažetak
Aim To analyze the neurotoxic potential of synthesized
magnetite nanoparticles coated by dextran, hydroxyethyl
starch, oxidized hydroxyethyl starch, and chitosan, and
magnetic nanoparticles combined with ferritin as a native
protein.
Methods The size of nanoparticles was analyzed using
photon correlation spectroscopy, their effects on the conductance
of planar lipid membrane by planar lipid bilayer
technique, membrane potential and acidification of synaptic
vesicles by spectrofluorimetry, and glutamate uptake
and ambient level of glutamate in isolated rat brain nerve
terminals (synaptosomes) by radiolabeled assay.
Results Uncoated synthesized magnetite nanoparticles
and nanoparticles coated by different polysaccharides had
no significant effect on synaptic vesicle acidification, the
initial velocity of L-[14C]glutamate uptake, ambient level
of L-[14C]glutamate and the potential of the plasma membrane
of synaptosomes, and conductance of planar lipid
membrane. Native ferritin-based magnetic nanoparticles
had no effect on the membrane potential but significantly
reduced L-[14C]glutamate transport in synaptosomes and
acidification of synaptic vesicles.
Conclusions Our study indicates that synthesized magnetite
nanoparticles in contrast to ferritin have no effects on
the functional state and glutamate transport of nerve terminals,
and so ferritin cannot be used as a prototype, analogue,
or model of polysaccharide-coated magnetic nanoparticle
in toxicity risk assessment and manipulation of
nerve terminals by external magnetic fields. Still, the ability
of ferritin to change the functional state of nerve terminals
in combination with its magnetic properties suggests its
biotechnological potential.
Ključne riječi
Hrčak ID:
129835
URI
Datum izdavanja:
15.6.2014.
Posjeta: 1.392 *