Veterinarski arhiv, Vol. 84 No. 6, 2014.
Izvorni znanstveni članak
Immunophenotyping of dendritic cells of the residing porcine gut-associated lymphoid tissues.
Gordan Mršić
; Center for Forensic Investigations, Research and Expertise „Ivan Vučetić“, Zagreb, Croatia
Vlado Godić
; Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Ana Kovšca Janjatović
; Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Katarina Špiranec
; Department of Anatomy, Histology and Embriology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Daniel Špoljarić
; Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Branka Gršković
; Center for Forensic Investigations, Research and Expertise „Ivan Vučetić“, Zagreb, Croatia
Josip Crnjac
; University Department for Forensic Sciences, University of Split, Split, Croatia
Damir Mihelić
; Department of Anatomy, Histology and Embriology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Maja Popović
; Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Sažetak
The aim of the study was to analyze the distribution patterns of porcine dendritic cells (DCs) of the residing mucosa of the ileum, caecum and colon, as well as mesenteric/colonic lymph nodes (MLN/CLN) of adult pigs. Three-color immunofluorescence (IF) was applied using monoclonal antibodies specific for porcine cell surface molecules (CD172, CD11R1 and CD16) on DCs. DCs were strategically localized within the lamina propria (LP) of the ileal villi, adjacent to the epithelial layer and around the crypts of the caecum/colon. Within the ileal LP, CD16+ and CD11R1+ cells were prevalent, whereas CD172+ cells were much rarer. Conversely, in the caecum the latter cells were more prominent, the CD16+ cells were also numerous, while CD11R1+ cells were extremely rare. In the colonic mucosa the most frequent were CD16+.CD11R1+ cells were very rare, whereas CD172+ cells were almost absent. In the MLN, CD172+ cells were prevalent within the follicular area, CD16+ cells were rare and none of the CD11R1+ cells were examined. Within the perifollicular area the most numerous were CD16+ cells, CD11R1+ cells were less prevalent, while CD172+ cells were sporadic. In the CLN, CD16+ cells were the most frequent in the perifollicular area, CD11R1+ cells were less frequent and none of the CD172+ cells were observed. Within the follicular area the most numerous were CD11R1+ cells, CD172+ cells were very rare and CD16+ cells were absent.
Ključne riječi
dendritic cells; gut-associated lymphoid tissues; pig
Hrčak ID:
130409
URI
Datum izdavanja:
5.12.2014.
Posjeta: 1.736 *