Veterinarski arhiv, Vol. 85 No. 1, 2015.
Izvorni znanstveni članak
Hematology parameters in dogs treated with subconjunctival application of limbal stem cells allografts.
Boris Pirkić
orcid.org/0000-0002-5137-9028
; Clinic of Surgery, Orthopaedics and Ophtalmology, Faculty of Veterinary Medicine, University of Zagreb, Croatia
Njetočka Gredelj Šimec
; Clinical Hospital Merkur, Zagreb, Croatia
Lea Slunjski
; Clinic of Surgery, Orthopaedics and Ophtalmology, Faculty of Veterinary Medicine, University of Zagreb, Croatia
Mirna Abbafy
; Clinic of Surgery, Orthopaedics and Ophtalmology, Faculty of Veterinary Medicine, University of Zagreb, Croatia
Dubravko Kezić
; Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Croatia
Daniel Špoljarić
; Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Croatia
Tomislav Babić
; Clinic of Surgery, Orthopaedics and Ophtalmology, Faculty of Veterinary Medicine, University of Zagreb, Croatia
Katarina Špiranec
; Department of Anatomy, Histology and Embriology, Faculty of Veterinary Medicine, University of Zagreb, Croatia
Gordan Mršić
; Center for Forensic Investigations, Research and Expertise „Ivan Vučetić“, Zagreb, Croatia
Damir Mihelić
; Department of Anatomy, Histology and Embriology, Faculty of Veterinary Medicine, University of Zagreb, Croatia
Dražen Matičić
; Clinic of Surgery, Orthopaedics and Ophtalmology, Faculty of Veterinary Medicine, University of Zagreb, Croatia
Maja Popović
; Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Croatia
Sažetak
The cornea, as a source for obtaining stem cells, is a good medium for their use in veterinary ophthalmology. In this research, we used dogs which had been diagnosed with extensive, chronic thinning of the central cornea (vertex corneae) generated due to complicated mechanical damage to the corneae pannosa. The goal of this study was to test whether subconjuctival application of limbal stem cells allografts had any influence on haematology parameters in peripheral blood. The animals were divided into three experimental groups regardless of breed, sex and age, and each group comprised 7 dogs. Group A consisted of clinically healthy dogs (control), in group B there were dogs who had had injuries treated by a conventional ophthalmological approach in veterinary medicine, and group C in which corneal injuries were treated using subconjuctival cell allografts. Blood samples were taken four times during the experiment from all animals for haematological analysis. Transplantation of limbal stem cell allografts did not affect the physiological haematology parameters.
Ključne riječi
dog; stem cell; limbus; veterinary ophthalmology
Hrčak ID:
135339
URI
Datum izdavanja:
23.2.2015.
Posjeta: 1.081 *