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Antibacterial Effects of Nd:Yag Laser in Root Canal Samples: In Vitro Study

Silvana Jukić
Ivana Miletić
Želimir Božić
Ivica Anić
Dragica Smoljak
Smilja Kalenić

Puni tekst: engleski pdf 78 Kb

str. 3-7

preuzimanja: 628



The aim of the study was to investigate the antibacterial effect of Nd:YAG laser in root canal samples using suspension of Enterococcus faecalis and Staphylococcus aureus. Twenty-two single-rooted teeth were instrumented, sterilized and inoculated with bacterial suspension concentration of 106 CFU/ml in brain-heart broth. The samples were irradiated by Nd:YAG laser, one half with power setting at 3.5 W, and the other half with 2.5 W, frequency of 40 Hz, in five cycles of 15-s lasing and 15-s pause for cooling. After irradiation, the samples were placed in vials with broth and the number of CFU/ml was determined after one and two weeks. Laser beams of 3.5 W power setting sterilized only three samples in the first week. In one of them, the bacteria predominated by Enterococcus faecalis grew in the second week, whereas pure culture of Enterococcus faecalis grew in another sample. Irradiation by Nd:YAG laser with 2.5 W did not sterilize any of the samples. Nd:YAG laser with the parameters used in this study did not significantly reduce the number of bacteria in root canal samples.

Ključne riječi

Root canal therapy - methods; Lasers - therapeutic use; Root canal preparation - instrumentation; Dental pulp cavity - microbiology; Disinfection - methods

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Podaci na drugim jezicima: hrvatski

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