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Indirect ELISA and Western blotting as tools to diagnose fascioloidosis in a population of free-ranging red deer (Cervus elaphus).

Krešimir Severin ; Department of Forensic and State Veterinary Medicine, Faculty of Veterinary Medicine University of Zagreb, Zagreb, Croatia
Franjo Martinković ; Department for Parasitology and Parasitic Diseases with Clinics, Faculty of Veterinary Medicine University of Zagreb, Zagreb, Croatia
Zdravko Janicki ; Department for Game Biology, Pathology and Breeding, Veterinary Faculty University of Zagreb, Zagreb, Croatia
Albert Marinculić ; Department for Parasitology and Parasitic Diseases with Clinics, Faculty of Veterinary Medicine University of Zagreb, Zagreb, Croatia
Alen Slavica ; Department for Game Biology, Pathology and Breeding, Veterinary Faculty University of Zagreb, Zagreb, Croatia
Diana Žele ; Institute for Breeding and Health Care of Wild Animals, Fishes and Bees, Faculty of Veterinary Medicine University of Ljubljana, Ljubljana, Slovenia
Gorazd Vengušt ; Institute for Breeding and Health Care of Wild Animals, Fishes and Bees, Faculty of Veterinary Medicine University of Ljubljana, Ljubljana, Slovenia
Petar Džaja ; Department of Forensic and State Veterinary Medicine, Faculty of Veterinary Medicine University of Zagreb, Zagreb, Croatia
Zdravka Vidić ; Laboratory for Diagnostic, Veterinary Institute Split, Croatian Veterinary Institute, Split, Croatia
Dean Konjević ; Department of Veterinary Economics and Analytical Epidemiology, Faculty of Veterinary Medicine University of Zagreb, Zagreb, Croatia


Puni tekst: engleski pdf 303 Kb

str. 563-576

preuzimanja: 491

citiraj


Sažetak

The aim of this research was to investigate the use of an indirect enzyme-linked immunosorbent assay (ELISA) and Western blotting test with native excretory/secretory Fascioloides magna antigen (ES-Ag) in free-ranging red deer (Cervus elaphus). The research was performed on 48 red deer shot during routine culls from two different areas, considering the occurrence of fascioloidosis. After coprological examination and gross pathology examination of the livers for F. magna, serum samples were divided into three groups as infected (n = 32), uninfected (n = 13) and previously infected deer (n = 3). Indirect ELISA results were significantly higher for serum samples from infected deer (percentage of positivity (PP) 65.1 ± 18.4) than uninfected (PP 11.6 ± 13.7) and previously infected deer (PP 20.3 ± 6.4). Samples from uninfected deer had fewer bands (30-33 and 104 kDa) on Western blotting than samples from infected deer (6, 17, 22, 27, 30-33, 40, 45 and 104 kDa). The number of alive flukes positively correlated with the ELISA results and the number of F. magna eggs per gram of faeces (EPG). The results indicated that ELISA and Western blotting test could be useful in the early diagnosis of fascioloidosis in red deer and also in control in the population of free-ranging red deer.

Ključne riječi

Fascioloides magna, red deer, excretory/secretory antigens, indirect ELISA, Western blotting

Hrčak ID:

148338

URI

https://hrcak.srce.hr/148338

Podaci na drugim jezicima: hrvatski

Posjeta: 992 *