Izvorni znanstveni članak

https://doi.org/10.1515/acph-2016-0022

High performance liquid chromatography for simultaneous determination of xipamide, triamterene and hydrochlorothiazide in bulk drug samples and dosage forms

SOAD S. ABD EL-HAY ; Faculty of Pharmacy, Pharmaceutical Chemistry Department, King Abdulaziz University, Saudi Arabia; Faculty of Pharmacy, Department of Analytical Chemistry, Zagazig University, Egypt
HISHAM HASHEM ; Faculty of Pharmacy, Department of Analytical Chemistry, Zagazig University, Egypt; Faculty of Pharmacy, Pharmaceutical Chemistry Department, Jazan University, Saudi Arabia
AYMAN A. GOUDA ; Faculty of Science, Department of Analytical Chemistry, Zagazig University, Egypt

Sažetak

A novel, simple and robust high-performance liquid chromatography (HPLC) method was developed and validated for simultaneous determination of xipamide (XIP), triamterene (TRI) and hydrochlorothiazide (HCT) in their bulk powders and dosage forms. Chromatographic separation was carried out in less than two minutes. The separation was performed on a RP C-18 stationary phase with an isocratic elution system consisting of 0.03 mol L–1orthophosphoric acid (pH 2.3) and acetonitrile (ACN) as the mobile phase in the ratio of 50:50 at 2.0 mL min–1 flow rate at room temperature. Detection was performed at 220 nm. Validation was performed concerning system suitability, limits of detection and quantitation, accuracy, precision, linearity and robustness. Calibration curves were rectilinear over the range of 0.195–100 µg mL–1 for all the drugs studied. Recovery values were 99.9, 99.6 and 99.0 % for XIP, TRI and HCT, respectively. The method was applied to simultaneous determination of the studied analytes in their pharmaceutical dosage forms.

Ključne riječi

xipamide; triamterene; hydrochlorothiazide; HPLC; simultaneous analysis

Hrčak ID:

153330

URI

https://hrcak.srce.hr/153330

Datum izdavanja:

31.3.2016.

Posjeta: 2.430 *