Izvorni znanstveni članak
Expression of Bcl-2 Family Proteins in Psoriasis.
Tanja Batinac
Gordana Zamolo
Ita Hadžisejdić
Gordana Žauhar
Gordana Brumini
Alen Ružić
Viktor Peršić
Sažetak
Aim To elucidate the mechanisms involved in apoptosis of psoriatic
keratinocytes by examining the expression of pro-apoptotic (Bak, Bax)
and anti-apoptotic (Bcl-2, Bcl-X) Bcl-2 family of proteins, as well as
the expression of p53 and Ki-67 proteins in normal skin, and uninvolved
and involved psoriatic skin.
Methods A total of 90 skin samples (30 cases of involved and uninvolved
psoriatic skin and normal skin) were examined immunohistochemicaly
to determine the protein expression of p53, Ki-67, Bcl-2,
Bcl-X, Bax, and Bak. The results were quantified and expressed as a
percentage of positive keratinocytes.
Results There was a significant increase in Ki-67 (17.05 vs 3.65;
P<0.001), Bcl-X (40.21 vs 13.97; P<0.001), Bak (89.46 vs 73.36;
P<0.001), and Bax (50.00 vs 29.25; P<0.001) expression and a decrease
in Bcl-2 (3.23 vs 6.25; P=0.008) expression in involved psoriatic skin,
as well as an increase in Bcl-X (25.13 vs 13.97; P<0.001) expression
in uninvolved psoriatic skin, when compared to normal skin. Samples
with higher percentage of Ki-67 positive cells showed a higher percentage
of p53 positive cells (correlation coefficient r = 0.75 in involved
psoriatic samples, P<0.001; r = 0.88 in uninvolved psoriatic samples,
P<0.001; and r = 0.85 in normal skin samples, P<0.001). Samples
with higher percentage of p53 positive cells expressed pro-apoptotic
Bak and Bax in higher percentage of cells; the correlation coefficients
were r = 0.74 and r = 0.68 in involved psoriatic samples (P<0.001 for
both), r = 0.75 and r = 0.69 in uninvolved psoriatic samples (P<0.001,
for both), and r = 0.87 and r = 0.70 in normal skin samples (P<0.001,
for both).
Conclusion Increased expression of Bcl-X protein was associated with
psoriatic epidermal hyperplasia. Strong Bax and Bak expression in involved
psoriatic skin are probably inhibitory mechanisms counteracting
intensive proliferation.
Ključne riječi
protein expression of p53; psoriatic skin
Hrčak ID:
16441
URI
Datum izdavanja:
20.6.2007.
Posjeta: 1.504 *