hrcak mascot   Srce   HID

Izvorni znanstveni članak
https://doi.org/10.2478/v10004-007-0018-2

Mechanistic Study on Aniline-Induced Erythrocyte Toxicity

Harpal Singh
Elissa Purnell
Carion Smith

Puni tekst: engleski, pdf (266 KB) str. 275-285 preuzimanja: 1.249* citiraj
APA 6th Edition
Singh, H., Purnell, E. i Smith, C. (2007). Mechanistic Study on Aniline-Induced Erythrocyte Toxicity. Arhiv za higijenu rada i toksikologiju, 58 (3), 275-285. https://doi.org/10.2478/v10004-007-0018-2
MLA 8th Edition
Singh, Harpal, et al. "Mechanistic Study on Aniline-Induced Erythrocyte Toxicity." Arhiv za higijenu rada i toksikologiju, vol. 58, br. 3, 2007, str. 275-285. https://doi.org/10.2478/v10004-007-0018-2. Citirano 15.06.2019.
Chicago 17th Edition
Singh, Harpal, Elissa Purnell i Carion Smith. "Mechanistic Study on Aniline-Induced Erythrocyte Toxicity." Arhiv za higijenu rada i toksikologiju 58, br. 3 (2007): 275-285. https://doi.org/10.2478/v10004-007-0018-2
Harvard
Singh, H., Purnell, E., i Smith, C. (2007). 'Mechanistic Study on Aniline-Induced Erythrocyte Toxicity', Arhiv za higijenu rada i toksikologiju, 58(3), str. 275-285. doi: https://doi.org/10.2478/v10004-007-0018-2
Vancouver
Singh H, Purnell E, Smith C. Mechanistic Study on Aniline-Induced Erythrocyte Toxicity. Arh Hig Rada Toksikol. [Internet]. 2007 [pristupljeno 15.06.2019.];58(3):275-285. doi: https://doi.org/10.2478/v10004-007-0018-2
IEEE
H. Singh, E. Purnell i C. Smith, "Mechanistic Study on Aniline-Induced Erythrocyte Toxicity", Arhiv za higijenu rada i toksikologiju, vol.58, br. 3, str. 275-285, 2007. [Online]. doi: https://doi.org/10.2478/v10004-007-0018-2

Sažetak
Strategies for the use of bio-indicators in the prediction of environmental damage should include mechanistic research. This study involves the relationship between the chemical structure and hemotoxic markers of aniline and its halogenated analogs. Aniline-induced methemoglobinemia, loss of circulating blood cells, blood stability, glutathione depletion and membrane cytoskeletal changes were assessed following exposure to phenylhydroxylamine (PHA), para-fluoro-, para-bromo-, and para-iodo in male Sprague-Dawley rats. Methemoglobin was determined spectrophotometrically at 635 nm. Erythrocyte depletion was investigated by loss of radioactivity in chromium-labeled red blood cells in vivo. Membrane proteins were analyzed by SDS-PAGE using red blood ghost cells treated with various aniline analogs. Results showed dose- and time-dependent changes in the induction of methemoglobin of up to 78 % with para-bromo PHA and 75 % with para-iodo PHA compared to 3 % to 5 % in control. Treated animals lost up to three times more blood from circulation compared to control within 14 days after treatment. Erythrocytes were more stable in buffer solution than in para-iodo-treated cells. Depletion of reduced glutathione in PHA and para-iodo-PHA treated red cells was also observed. Analysis of red cell skeletal membrane treated with para-iodo-PHA showed that protein band 2.1 became broader and band 2.2 diminished completely in some treatments. Dose- and time-dependent changes suggested the use of hemotoxic endpoints as potential biomarkers for assessing chemical and drug safety

Ključne riječi
cytoskeletal proteins; hemolytic anemia; hemotoxicity; methemoglobin; para-bromo-PHA; para-fluoro-PHA; para-iodo-PHA; phenylhydroxylamine

Hrčak ID: 16521

URI
https://hrcak.srce.hr/16521

[hrvatski]

Posjeta: 1.726 *