Veterinarski arhiv, Vol. 86 No. 5, 2016.
Izvorni znanstveni članak
Relationship between paraoxonase-1 activity and lipid mobilisation in transition dairy cows.
Romana Turk
; Department of Pathophysiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Ivan Folnožić
; Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Dražen Đuričić
; Veterinary Practice Đurđevac, Đurđevac, Croatia
Silvijo Vince
; Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Zlata Flegar-Meštrić
; Department of Medical Biochemistry and Laboratory Medicine, Merkur University Hospital, Zagreb, Croatia
Tomislav Dobranić
; Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Hrvoje Valpotić
; Department for Nutrition and Animal Husbandry, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Marko Samardžija
; Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Sažetak
The objectives of this study were to investigate the influence of the
transition period on lipid mobilisation and paraoxonase-1 (PON1) activity, as well as the relationship between the indicators of lipid metabolism and PON1 activity. Twenty-four Holstein-Friesian dairy cows (aged 2-7 years) were included in the study. Parameters of lipid metabolism (triglycerides, total cholesterol, HDL-C, NEFA and BHB) and PON1 activity were monitored on days -30, -10, -2, 0, 5, 12, 19, 26 and 60 relative to parturition. Triglyceride concentration was significantly decreased from parturition until day 60 after calving, as compared to the values obtained before calving (P<0.05). Both total cholesterol and HDL-C concentrations significantly decreased at calving, with an increase during lactation (P<0.05). Serum NEFA concentrations significantly increased at calving (P<0.05) and stayed at the highest values up to day 19 after calving. Serum BHB concentrations increased significantly after calving on days 12 and 19 (P<0.05), which may be a consequence of increased NEFA around parturition. These changes indicate fat mobilisation from adipose tissue due to the energy deficit during the transition period. Serum PON1 activity decreased at calving but increased significantly on days 26 and 60 postpartum, suggesting a reduced antioxidant status in the postpartum period. Additionally, PON1 significantly positively correlated with total cholesterol (r = 0.42) and HDL-C (r = 0.49) and inversely correlated with NEFA (r = -0.33). The results suggested that PON1 activity is related to lipid metabolism and lipomobilisation syndrome and could be considered as a putative marker for metabolic and inflammatory-related disorders in transition dairy cows.
Ključne riječi
non-esterified fatty acids; β-hydroxybutirate; negative energy balance; oxidative stress; inflammation
Hrčak ID:
167363
URI
Datum izdavanja:
23.9.2016.
Posjeta: 1.059 *