Veterinarski arhiv, Vol. 86 No. 5, 2016.
Izvorni znanstveni članak
The genetic heterogeneity of equine infectious anaemia virus field strains in Croatia.
Vladimir Stevanović
; Department of Microbiology and Infectious Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Ivana Lojkić
; Department of Virology, Croatian Veterinary Institute, Zagreb, Croatia
Ljubo Barbić
; Department of Microbiology and Infectious Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Snježana Kovač
; Department of Microbiology and Infectious Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Vesna Mojčec-Perko
; Department of Microbiology and Infectious Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Andreja Ambriović-Ristov
; Laboratory for Genotoxic Agents, Division of Molecular Biology, Ruđer Bošković Institute, Zagreb, Croatia
Suzana Hađina
; Department of Microbiology and Infectious Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Lejla Velić
; Department of Epizootiology, Faculty of Veterinary Medicine, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
Matko Perharić
Vilim Starešina
; Department of Microbiology and Infectious Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Sažetak
Equine infectious anaemia (EIA) is an infectious disease of equids caused by lentivirus. Equine infectious anaemia virus (EIAV) causes lifelong infection after integration of the proviral DNA into the host cell genome. Due to the obligatory slaughter of infected animals in most countries of the world, EIA is one of the most significant infectious diseases of horses. Control of EIA is based on antibody detection, but today the focus in diagnostics is aimed at PCR based methods. In this study, 98 AGID (agar gel immunodiffusion) positive sera samples, three spleen samples and cell cultures infected with EIA were tested for the presence of viral RNA or proviral DNA, using two nested PCR protocols. Out of all the samples 27 partial gag sequences of viral RNA were amplified. The Croatian isolates showed high diversity within the group of other available European sequences. Phylogenetic analysis showed that at least three gag gene subtypes are presently circulating in Croatia. The results of this study showed the limited efficiency of two PCR methods in terms of diagnostics, although the primers used for amplification targeted part of the viral RNA that was regarded as one of the most preserved. The high variability of the Croatian isolates obtained highlighted the necessity for determining more EIAV sequences to allow detection of less variable areas of the viral genome, to be used for amplification with universal primers.
Ključne riječi
equine infectious anaemia; diagnostics; field isolates; phylogenetic analysis; Croatia
Hrčak ID:
167467
URI
Datum izdavanja:
23.9.2016.
Posjeta: 1.792 *