Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2016.57.51
Hyperbaric oxygen preconditioning ameliorates blood-brain barrier damage induced by hypoxia through modulation of tight junction proteins in an in vitro model
Lei Hao
; Department of Neurology, No 324 Hospital of People’s LiberationArmy, Chongqing, China
Xiuming Guo
; Department of Neurology The First Affiliated Hospital of Chongqing Medical University,Chongqing, China
Can Zou
; Department of Neurology, No 324 Hospital of People’s LiberationArmy, Chongqing, China
Huchuan Zhou
; Department of Neurology, No 324 Hospital of People’s Liberation Army, Chongqing, China
Hong Tian
; Department of Neurology, No 324 Hospital of People’s Liberation Army, Chongqing, China
Yubo Zhang
; Department of Neurology, No 324 Hospital of People’s Liberation Army, Chongqing, China
Chuan Song
; Department of Neurology, No 324 Hospital of People’s Liberation Army, Chongqing, China
Lei Liu
; Department of Neurology, No 324 Hospital of People’s Liberation Army, Chongqing, China
Sažetak
Aim To explore the effects of hyperbaric oxygen preconditioning
(HBOP) on the permeability of blood-brain barrier
(BBB) and expression of tight junction proteins under hypoxic
conditions in vitro.
Methods A BBB in vitro model was constructed using the
hCMEC/D3 cell line and used when its trans-endothelial
electrical resistance (TEER) reached 80-120 Ω · cm2 (tested
by Millicell-Electrical Resistance System). The cells were
randomly divided into the control group cultured under
normal conditions, the group cultured under hypoxic conditions
(2%O2) for 24 h (hypoxia group), and the group
first subjected to HBOP for 2 h and then to hypoxia (HBOP
group). Occludin and ZO-1 expression were analyzed by
immunofluorescence assay.
Results Normal hCMEC/D3 was spindle-shaped and
tightly integrated. TEER was significantly reduced in the
hypoxia (P = 0.001) and HBOP group (P = 0.014) compared
to control group, with a greater decrease in the hypoxia
group. Occludin membranous expression was significantly
decreased in the hypoxia group (P = 0.001) compared to
the control group, but there was no change in the HBOP
group. ZO-1 membranous expression was significantly decreased
(P = 0.002) and cytoplasmic expression was significantly
increased (P = 0.001) in the hypoxia group compared
to the control group, although overall expression
levels did not change. In the HBOP group, there was no
significant change in ZO-1 expression compared to the
control group.
Conclusion Hyperbaric oxygen preconditioning protected
the integrity of BBB in an in vitro model through modulation
of occludin and ZO-1 expression under hypoxic conditions.
Ključne riječi
Hrčak ID:
169153
URI
Datum izdavanja:
15.2.2016.
Posjeta: 918 *