Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2016.57.371
Angiotensin-converting enzyme inhibitors reduce oxidative stress intensity in hyperglicemic conditions in rats independently from bradykinin receptor inhibitors
Kinga Mikrut
; Department of Physiology, Poznan University of Medical Sciences, Poznań, Poland
Justyna Kupsz
; Department of Physiology, Poznan University of Medical Sciences, Poznań, Poland
Jacek Koźlik
; Department of Physiology, Poznan University of Medical Sciences, Poznań, Poland
Hanna Krauss
; Department of Physiology, Poznan University of Medical Sciences, Poznań, Poland
Ewa Pruszyńska-Oszmałek
; Department of Animal Physiology and Biochemistry, Poznan University of Life Sciences, Poznań,Poland
Magdalena Gibas-Dorna
; Department of Physiology, Poznan University of Medical Sciences, Poznań, Poland
Sažetak
Aim To investigate whether bradykinin-independent antioxidative
effects of angiotensin-converting enzyme inhibitors
(ACEIs) exist in acute hyperglycemia.
Methods Male Wistar rats were divided into the normoglycemic
group (n = 40) and the hyperglycemic group
(n = 40). Hyperglycemia was induced by a single intraperitoneal
injection of streptozotocin (STZ, 65 mg/kg body
weight), dissolved in 0.1 mol/L citrate buffer (pH 4.5), 72
hours before sacrifice. The normoglycemic group received
the same volume of citrate buffer. Each group was divided
into five subgroups (n = 8): control group, captopril group,
captopril + bradykinin B1 and B2 receptor antagonists
group, enalapril group, and enalapril + bradykinin B1 and
B2 receptor antagonists group. Captopril, enalapril, B1 and
B2 receptor antagonists, or 0.15 mol/L NaCl were given at
2 and 1 hour before sacrifice. Oxidative status was determined
by measuring the concentration of malondialdehyde
and H2O2, and the activity of superoxide dismutase
(SOD), catalase (CAT), and glutathione peroxidase (GPx).
Results In STZ-induced hyperglycemic rats ACEIs significantly
reduced H2O2 and MDA concentration, while they
significantly enhanced SOD and GPx activity. The hyperglycemic
group treated simultaneously with ACEIs and bradykinin
B1 and B2 receptor antagonists showed a significant
decrease in H2O2 concentration compared to the control
hyperglycemic group.
Conclusion These results suggest the existence of additional
antioxidative effect of ACEIs in hyperglycemic conditions,
which is not related to the bradykinin mediation and
the structure of the drug molecule.
Ključne riječi
Hrčak ID:
170341
URI
Datum izdavanja:
15.8.2016.
Posjeta: 1.250 *