Izvorni znanstveni članak
https://doi.org/10.1515/aiht-2016-67-2851
Micronuclei and other nuclear anomalies in buccal epithelial cells of children with chronic kidney disease
Banu Aykanat
; Department of Toxicology, Faculty of Pharmacy, Faculty of Medicine, Baskent University, Ankara, Turkey
Gonca Cakmak Demircigil
; Department of Toxicology, Faculty of Pharmacy, Faculty of Medicine, Baskent University, Ankara, Turkey
Necla Buyan
; Department of Pediatric Nephrology, Faculty of Medicine, Baskent University, Ankara, Turkey
Esra Baskin
; Faculty of Medicine, Gazi University, Department of Pediatric Nephrology, Faculty of Medicine, Baskent University, Ankara, Turkey
Kaan Gulleroglu
; Faculty of Medicine, Gazi University, Department of Pediatric Nephrology, Faculty of Medicine, Baskent University, Ankara, Turkey
Kibriya Fidan
; Department of Pediatric Nephrology, Faculty of Medicine, Baskent University, Ankara, Turkey
Umut Selda Bayrakci
; Faculty of Medicine, Gazi University, Department of Pediatric Nephrology, Faculty of Medicine, Baskent University, Ankara, Turkey
Aydin Dalgic
; Department of General Surgery, Faculty of Medicine, Baskent University, Ankara, Turkey
Hamdi Karakayali
; Department of General Surgery, Faculty of Medicine, Baskent University, Ankara, Turkey
Mehmet Haberal
; Department of General Surgery, Faculty of Medicine, Baskent University, Ankara, Turkey
Sema Burgaz
; Department of Toxicology, Faculty of Pharmacy, Faculty of Medicine, Baskent University, Ankara, Turkey
Sažetak
The objective of this study was to reveal the likely genomic instability in children with chronic kidney disease (CKD) using micronucleus (MN) assay on buccal epithelial cells (BEC). We investigated the frequencies of micronuclei and other nuclear anomalies, such as nuclear buds, binucleated cells, condensed chromatin, and karyorrhectic and pyknotic cells in BEC. Children with CKD were grouped as follows: children in the pre-dialysis (PreD) stage (N=17), children on regular haemodialysis (HD) (N=14), and children who have undergone transplantation (Tx) (N=17). As a control group, twenty age- and gender-matched healthy children were selected. The MN frequency in BEC of all groups of children with CKD was significantly elevated (5- to 7-fold) as compared to the control group (p<0.001). In contrast, the frequencies of nuclear buds were not significantly higher in the study groups compared to the control group. The frequencies of binucleated cells and condensed chromatin cells were significantly higher in all subgroups of children with CKD relative to the control group (p<0.001). Our results show that the BEC of pediatric PreD, HD, and Tx patients with CKD display increased cytogenetic, cytokinetic, and cytotoxic effects. They also point to the sensitivity and usefulness of the BEC MN assay in the assessment of genetic susceptibility of patients with CKD.
Ključne riječi
buccal micronucleus assay; genotoxicity; dialysis; renal ransplantation; nuclear anomalies
Hrčak ID:
170515
URI
Datum izdavanja:
14.12.2016.
Posjeta: 1.682 *