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Immune responses of BALB/c mice orally immunized with Salmonella Typhimurium ghost cells carrying antigens of enterotoxigenic Escherichia coli

Se W. Kim ; Division of Applied Life Sciences (BK21 & EBNCRC), Graduate School of Gyeongsang National University, Jinju, South Korea
Sang W. Gal ; Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, Jinju, South Korea
John H. Lee ; College of Veterinary Medicine, Chonbuk National University, Iksan Campus, Iksan, South Korea
Young-Sool Hah ; Clinical Research Institute, Gyeongsang National University Hospital, Jinju, South Korea
Tae W. Kim ; Swine Science and Technology Center, Gyeongnam National University of Science and Technology, Jinju, South Korea
Chul W. Kim ; Swine Science and Technology Center, Gyeongnam National University of Science and Technology, Jinju, South Korea
Il-Suk Kim ; Swine Science and Technology Center, Gyeongnam National University of Science and Technology, Jinju, South Korea
Sam W. Kim ; Swine Science and Technology Center, Gyeongnam National University of Science and Technology, Jinju, South Korea

Sažetak

Salmonella Typhimurium ghost vaccines containing antigens of enterotoxigenic Escherichia coli (ETEC) were obtained by a strain harboring pMMP184, which carries a ghost cassette. The immune responses of BALB/c mice orally vaccinated with these ghost vaccines were determined in this study. Total IgG against S. Typhimurium were highly detected by the oral immunization route in BALB/c mice. IgGs against ETEC antigens in the ghost cells carrying F41 and intimin were detected at 4 weeks after vaccination. However, FedF elicited a delayed induction of IgGs, whereas FedA immune response failed to or barely induced IgGs after vaccination. Proliferations of CD3e/CD4-T cells were observed in splenocytes of BALB/c mice immunized with S. Typhimurium ghost cells carrying FedF. However, CD45R-B220/CD23-B cells were proliferated by ghost cells carrying FedA, F41, and intimin. The immunized BALB/c mice showed 25~50% protection against challenge with wild type S. Typhimurium, when compared to control mice. Therefore, it is assumed that oral vaccination of S. Typhimurium ghost cells has the potential to protect mice against pathogenic E. coli.

Ključne riječi

antigen: BALB/c mouse: ghost cells; pMMP184; S. Typhimurium; vaccination

Hrčak ID:

178532

URI

https://hrcak.srce.hr/178532

Datum izdavanja:

15.1.2017.

Podaci na drugim jezicima: hrvatski

Posjeta: 937 *