Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2016.57.540
Assessment of apoptosis in the native vein used for hemodialysis access
Laura Leci-Tahiri
; Clinic of Vascular Surgery University Clinical Center of Kosovo,Prishtina, Kosovo
Božo Krušlin
; Ljudevit Jurak” Department of Pathology, University Hospital
Majda Vučić
; Ljudevit Jurak” Department of Pathology, University Hospital
Zdenko Sonicki
; Department of Medical Statistics Epidemiology, and MedicalInformatics, Andrija Štampar Schoolof Public Health, University ofZagreb School of Medicine, Zagreb, Croatia
Ivo Lovričević
; Department of Vascular Surgery, University Hospital Center “Sestremilosrdnice,” Zagreb, Croatia
Sažetak
Aim To determine whether apoptosis is more common in
previously punctured native veins than in non-punctured
native veins among patients who undergo surgical creation
of arteriovenous fistula (AVF) for dialysis access.
Methods Cephalic vein specimens were obtained from
January 1, 2013 to December 31, 2014 from 60 patients, 30
with previously punctured native veins and 30 with nonpunctured
native veins. Before AVF placement, a 1-cm vein
segment was excised from distal part of the vein for histological,
histochemical, and immunohistochemical analysis.
Vein specimens were divided into two portions along the
longitudinal axis and stained with hematoxylin and eosin
for routine histological evaluation. Immunohistochemical
analysis was used to localize Bax, p53, caspase 3, and Bcl-2
expression.
Results The group with previously punctured veins
showed significantly increased caspase 3 (P < 0.001, twosided
Fisher`s Exact Test) and Bax expression (P = 0.002,
two-sided Fisher`s Exact Test) and significantly decreased
Bcl-2 expression (P < 0.001, two-sided Fisher`s Exact Test)
compared with the control group. There were no significant
differences between the groups in p53 expression
(χ2 = 0.071, df = 1, P = 0.791). Fistula failure was significantly
more common in the study group (26.7% vs 6.7%, χ2 = 4.32,
df = 1, P = 0.038).
Conclusion Our study indicates a possible role of venipuncture
in apoptosis development and a possible role of
apoptosis in fistula failure, but we do not have sufficient
evidence to conclude that it represents its main cause.
Ključne riječi
Hrčak ID:
181393
URI
Datum izdavanja:
15.12.2016.
Posjeta: 892 *