Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2016.57.566
Genetic variation in leptin and leptin receptor genes is a risk factor for idiopathic recurrent spontaneous abortion
Andrijana Müller
; Department of Gynecology and Obstetrics, University Hospital Center Osijek, Osijek, Croatia
Jasenka Wagner
; Department of Medical Biology and Genetics, Faculty of MedicineJosip Juraj Strossmayer University of Osijek, Osijek, Croatia
Alenka Hodžić
; Clinical Institute of Medical Genetics, Division of Obstetrics and Gynecology, University Medical Center Ljubljana, Ljubljana, Slovenia
Aleš Maver
; Clinical Institute of Medical Genetics, Division of Obstetrics and Gynecology, University Medical Center Ljubljana, Ljubljana, Slovenia
Ivana Škrlec
; Department of Medical Biology and Genetics, Faculty of MedicineJosip Juraj Strossmayer University of Osijek, Osijek, Croatia
Marija Heffer
; Department of Medical Biology and Genetics, Faculty of MedicineJosip Juraj Strossmayer University of Osijek, Osijek, Croatia
Lada Zibar
; Department for Dialysis, Internal Clinic, University Hospital Center Osijek, Osijek, Croatia
Borut Peterlin
orcid.org/0000-0001-7824-4978
; Clinical Institute of Medical Genetics, Division of Obstetricsand Gynecology, UniversityMedical Center Ljubljana, Ljubljana, Slovenia
Sažetak
Aim To determine whether maternal leptin (LEP) and leptin
receptor (LEPR) gene polymorphisms are associated
with idiopathic recurrent spontaneous abortion (IRSA).
Methods This case-control association study conducted
from 2010 to 2012 at the Department of Gynecology and
Obstetrics, University Hospital Center Osijek and Clinical Institute
of Medical Genetics Ljubljana included 178 women
with a history of three or more IRSAs before the 22nd week
of gestation and 145 women with at least two live births
and no history of pathologic pregnancies during reproductive
period. Polymorphisms of maternal LEP (rs7799039,
rs2122627, rs11761556, rs10244329) and LEPR (rs1137101,
rs7516341, rs1186403, rs12062820) were assessed by allele
specific real-time polymerase chain reaction. Genotype
distribution, allele frequencies, and frequency of haplotypes
at LEP and LEPR genetic loci were determined.
Results We observed more frequent genotype for
rs7516341 (nominal P = 0.034, odds ratio [OR] 0.61, 95%
confidence interval [CI] 0.38-0.97) and rs1137101 (nominal
P = 0.048, OR 1.66, 95% CI 1.00-2.80) in the LEPR gene in
patients than in controls, but these results did not remain
significant after correction for multiple testing according
to Bonferroni (adjusted P value threshold was set at 0.05).
We did not observe differential distribution of genotype
frequencies in the LEP gene between cases and controls. In
patients with IRSA, GTCC haplotype in the LEPR gene locus
was significantly less frequent than in controls (P = 0.00865,
OR 0.45), contrary to ACTC haplotype (P = 0.0087, OR 1.98).
Conclusions We showed that genetic variability in the
LEPR gene was associated with IRSA, warranting confirmation
on a greater number of patients and pathogenesis investigation.
Ključne riječi
Hrčak ID:
181396
URI
Datum izdavanja:
15.12.2016.
Posjeta: 1.510 *