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https://doi.org/10.5562/cca3135

Kinetic Spectrophotometric Determination of N-Acetyl-L-cysteine Ethyl Ester (NACET) Generating Chromogenic Copper(I)Ln Complexes with Different Ligands

Lea Kukoc-Modun   ORCID icon orcid.org/0000-0003-1798-6796 ; Department of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, HR-21000 Split, Croatia
Dimitrios Tsikas ; Center of Pharmacology and Toxicology, Hannover Medical School, Carl-Neuberg-Str. 1, DE-30625 Hannover, Germany
Tomislav Kraljević ; Department of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, HR-21000 Split, Croatia
Maja Biocic   ORCID icon orcid.org/0000-0002-2293-3480 ; Department of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, HR-21000 Split, Croatia
Njegomir Radić ; Department of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, HR-21000 Split, Croatia

Puni tekst: engleski, pdf (6 MB) str. 263-271 preuzimanja: 322* citiraj
APA 6th Edition
Kukoc-Modun, L., Tsikas, D., Kraljević, T., Biocic, M. i Radić, Nj. (2017). Kinetic Spectrophotometric Determination of N-Acetyl-L-cysteine Ethyl Ester (NACET) Generating Chromogenic Copper(I)Ln Complexes with Different Ligands. Croatica Chemica Acta, 90 (2), 263-271. https://doi.org/10.5562/cca3135
MLA 8th Edition
Kukoc-Modun, Lea, et al. "Kinetic Spectrophotometric Determination of N-Acetyl-L-cysteine Ethyl Ester (NACET) Generating Chromogenic Copper(I)Ln Complexes with Different Ligands." Croatica Chemica Acta, vol. 90, br. 2, 2017, str. 263-271. https://doi.org/10.5562/cca3135. Citirano 20.10.2019.
Chicago 17th Edition
Kukoc-Modun, Lea, Dimitrios Tsikas, Tomislav Kraljević, Maja Biocic i Njegomir Radić. "Kinetic Spectrophotometric Determination of N-Acetyl-L-cysteine Ethyl Ester (NACET) Generating Chromogenic Copper(I)Ln Complexes with Different Ligands." Croatica Chemica Acta 90, br. 2 (2017): 263-271. https://doi.org/10.5562/cca3135
Harvard
Kukoc-Modun, L., et al. (2017). 'Kinetic Spectrophotometric Determination of N-Acetyl-L-cysteine Ethyl Ester (NACET) Generating Chromogenic Copper(I)Ln Complexes with Different Ligands', Croatica Chemica Acta, 90(2), str. 263-271. https://doi.org/10.5562/cca3135
Vancouver
Kukoc-Modun L, Tsikas D, Kraljević T, Biocic M, Radić Nj. Kinetic Spectrophotometric Determination of N-Acetyl-L-cysteine Ethyl Ester (NACET) Generating Chromogenic Copper(I)Ln Complexes with Different Ligands. Croatica Chemica Acta [Internet]. 2017 [pristupljeno 20.10.2019.];90(2):263-271. https://doi.org/10.5562/cca3135
IEEE
L. Kukoc-Modun, D. Tsikas, T. Kraljević, M. Biocic i Nj. Radić, "Kinetic Spectrophotometric Determination of N-Acetyl-L-cysteine Ethyl Ester (NACET) Generating Chromogenic Copper(I)Ln Complexes with Different Ligands", Croatica Chemica Acta, vol.90, br. 2, str. 263-271, 2017. [Online]. https://doi.org/10.5562/cca3135

Sažetak
Three simple, sensitive and robust kinetic spectrophotometric methods for the determination of a novel lipophilic thiol compound, N-acetyl-L-cysteine ethyl ester (NACET), have been developed and validated. The methods are based on the reduction of Cu(II)-ligand complex to Cu(I)-ligand complex with the analyte. Studied ligands were neocuproine, bicinchoninic acid (BCA) and bathocuproine disulfonic acid (BCS). The development of chromogenic complexes was followed using kinetic setup with spectrophotometric detection at 458, 562 and 483 nm for the reactions of NACET with neocuproine, BCA and BCS, respectively. The calculated reaction orders with respect to NACET concentration were found to be 1.07, 1.01, 1.07, respectively, thus confirming a first order of reaction. The initial rate and fixed time methods were utilized for constructing calibration curves. Assays limits of detection were 1.4 × 10–7, 3.2 × 10−7 and 6.0 × 10−8 mol L–1, respectively. The analytical performance of the methods, in terms of accuracy and precision, was established.

Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 International License.

Ključne riječi
N-acetyl-L-cysteine ethyl ester; kinetic spectrophotometry; neocuproine; bicinchoninic acid; bathocuproine disulfonic acid

Hrčak ID: 185729

URI
https://hrcak.srce.hr/185729

Posjeta: 448 *