Izvorni znanstveni članak

https://doi.org/10.2478/acph-2018-0005

Induction of xenobiotic-metabolizing enzymes in hepatocytes by beta-naphthoflavone: Time-dependent changes in activities, protein and mRNA levels

KATEŘINA LNĚNIČKOVÁ ; Faculty of Pharmacy, Charles University, CZ-500 05 Hradec Králové, Czech Republic
LENKA SKÁLOVÁ ; Faculty of Pharmacy, Charles University, CZ-500 05 Hradec Králové, Czech Republic
LUCIE STUCHLÍKOVÁ RAISOVÁ ; Faculty of Pharmacy, Charles University, CZ-500 05 Hradec Králové, Czech Republic
BARBORA SZOTÁKOVÁ ; Faculty of Pharmacy, Charles University, CZ-500 05 Hradec Králové, Czech Republic
PETRA MATOUŠKOVÁ ; Faculty of Pharmacy, Charles University, CZ-500 05 Hradec Králové, Czech Republic

Sažetak

In the present study, time-dependency of the induction effect of a selective inducer on the activity, protein and mRNA levels of cytochromes P450 1A1/2 (CYP1A1/2), NAD(P)H:quinone oxidoreductase 1 (NQO1) and glutathione S-transferases (GSTA), in primary culture of rat hepatocytes was tested and evaluated. To show the differences in responses of tested enzymes, the common aryl hydrocarbon receptor (AhR) ligand agonist, beta-naphthoflavone (BNF), was used. Induction of CYP1A1/2 by BNF was detected at all time intervals and at all levels (i.e., mRNA, protein, enzyme activity). Different responses of NQO1 and GSTA upon BNF treatment were observed. Our results demonstrate that the responses of different xenobiotic-metabolizing enzymes to the inducer vary in time and depend on the measured parameter. For these reasons, an induction study featuring only one-time interval treatment and/or one parameter testing could produce misleading information.

Ključne riječi

aryl hydrocarbon receptor (AhR); beta-naphthoflavone; rat hepatocyte; time dependency; mRNA-protein correlation

Hrčak ID:

189687

URI

https://hrcak.srce.hr/189687

Datum izdavanja:

31.3.2018.

Posjeta: 1.682 *