Izvorni znanstveni članak
https://doi.org/10.15255/CABEQ.2016.930
Efficient Production of Exopolysaccharide by Submerged Fermentation of Hypsizygus marmoreus Using a Two-stage pH Control Strategy
Y. Chen
; School of Life & Environmental Science, Wenzhou University, Chashan University Town, Wenzhou 325035, China
X. Y. Zhang
; School of Life & Environmental Science, Wenzhou University, Chashan University Town, Wenzhou 325035, China
G. N. Xiao
; Zhejiang Provincial Key Laboratory for Chemical and Biological Processing Technology of Farm Products, Zhejiang University of Science and Technology, 318 Liuhe Road, Hangzhou 310023, China
H. L. Yang
orcid.org/0000-0003-0666-9955
; School of Life & Environmental Science, Wenzhou University, Chashan University Town, Wenzhou 325035, China
Sažetak
The effects of culture conditions and pH values on the exopolysaccharides (EPS) production by Hypsizygus marmoreus were investigated in this work. The results showed that the optimal carbon and nitrogen sources were glucose and yeast extract at concentrations of 30 g L–1 and 12.5 g L–1, respectively. In constant pH fermentation, high biomass concentration and the specific cell growth rate were achieved at pH 6.5, while high EPS concentration and the specific EPS production rate were obtained at pH 7.5, respectively. Based on kinetics analysis, a two-stage pH strategy was proposed in which pH was controlled at 6.5 to maintain high cell growth and glucose consumption in the first 48 h, and then shifted to 7.5 for increasing EPS production. The maximal EPS concentration
reached 2.31 ± 0.07 g L–1 by applying this strategy. The EPS productivity (0.024 g L–1 h–1) increased 58.94 %, 172.7 % and 35.73 %, respectively, compared with that of the natural pH, and controlling pH at 6.5 and 7.5.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Ključne riječi
Hypsizygus marmoreus; exopolysaccharide; submerged fermentation; kinetics analysis; two-stage pH strategy
Hrčak ID:
192356
URI
Datum izdavanja:
10.1.2018.
Posjeta: 1.078 *