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Izvorni znanstveni članak
https://doi.org/10.5562/cca3343
The Mechanism of Peptide Hydrolysis Catalysed by Dipeptidyl Peptidase III from Bacteroides thetaiotaomicron
Marko Tomin
orcid.org/0000-0001-6894-6810
; Division of Organic Chemistry and Biochemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia
Antonija Tomić
; Division of Organic Chemistry and Biochemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia
Borislav Kovačević
; Division of Physical Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia
Sanja Tomić
orcid.org/0000-0002-0550-0878
; Division of Organic Chemistry and Biochemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia
| Puni tekst: engleski, pdf (3 MB) | str. 187-193 | citiraj | |
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APA 6th Edition Tomin, M., Tomić, A., Kovačević, B. i Tomić, S. (2018). The Mechanism of Peptide Hydrolysis Catalysed by Dipeptidyl Peptidase III from Bacteroides thetaiotaomicron. Croatica Chemica Acta, 91 (2), 187-193. https://doi.org/10.5562/cca3343 MLA 8th Edition Tomin, Marko, et al. "The Mechanism of Peptide Hydrolysis Catalysed by Dipeptidyl Peptidase III from Bacteroides thetaiotaomicron." Croatica Chemica Acta, vol. 91, br. 2, 2018, str. 187-193. https://doi.org/10.5562/cca3343. Citirano 04.03.2021. Chicago 17th Edition Tomin, Marko, Antonija Tomić, Borislav Kovačević i Sanja Tomić. "The Mechanism of Peptide Hydrolysis Catalysed by Dipeptidyl Peptidase III from Bacteroides thetaiotaomicron." Croatica Chemica Acta 91, br. 2 (2018): 187-193. https://doi.org/10.5562/cca3343 Harvard Tomin, M., et al. (2018). 'The Mechanism of Peptide Hydrolysis Catalysed by Dipeptidyl Peptidase III from Bacteroides thetaiotaomicron', Croatica Chemica Acta, 91(2), str. 187-193. https://doi.org/10.5562/cca3343 Vancouver Tomin M, Tomić A, Kovačević B, Tomić S. The Mechanism of Peptide Hydrolysis Catalysed by Dipeptidyl Peptidase III from Bacteroides thetaiotaomicron. Croatica Chemica Acta [Internet]. 2018 [pristupljeno 04.03.2021.];91(2):187-193. https://doi.org/10.5562/cca3343 IEEE M. Tomin, A. Tomić, B. Kovačević i S. Tomić, "The Mechanism of Peptide Hydrolysis Catalysed by Dipeptidyl Peptidase III from Bacteroides thetaiotaomicron", Croatica Chemica Acta, vol.91, br. 2, str. 187-193, 2018. [Online]. https://doi.org/10.5562/cca3343 |
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Sažetak
Dipeptidyl peptidase III (DPP III) is a zinc-dependent peptidase that cleaves dipeptides off of N-termini of its substrates. Previous studies on human DPP III reveal a reaction mechanism similar to that of thermolysin. Since the active site is conserved within the DPP III family, it is not surprising that the mechanism determined for Bacteroides thetaiotaomicron DPP III (BtDPP III) closely resembles that of hDPP III. However, the hydrogen bond network within the model differs slightly from that in the human ortholog, which results in two proposed pathways. The calculated Gibbs activation energy of 90.1 kJ mol–1 is larger than the one calculated from kinetic data for the preferred substrate Arg2-2-naphthylamide at room temperature (69 kJ mol–1), suggesting the importance of treating the whole DPP III enzyme in the calculations.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Ključne riječi
dipeptidyl peptidase III; DPP III; peptide hydrolysis; reaction mechanism; Bacteroides thetaiotaomicron
Hrčak ID: 201073
URI
https://hrcak.srce.hr/201073
Moj profil
Srce