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https://doi.org/10.15255/CABEQ.2018.1335

Shedding Light on Lipase Stability in Natural Deep Eutectic Solvents

A. A. Elgharbawy ; International Institute for Halal Research and Training (INHART), International Islamic University Malaysia, Kuala Lumpur 50728, Malaysia
A. Hayyan orcid id orcid.org/0000-0002-3738-8198 ; b)Nanotechnology & Catalysis Research Centre (NANOCAT), University of Malaya, Kuala Lumpur 50603, Malaysia; c)University of Malaya Centre for Ionic Liquids (UMCiL), University of Malaya, Kuala Lumpur 50603, Malaysia
M. Hayyan orcid id orcid.org/0000-0002-2179-0801 ; c)University of Malaya Centre for Ionic Liquids (UMCiL), University of Malaya, Kuala Lumpur 50603, Malaysia; d)Department of Chemical Engineering, Faculty of Engineering, Sohar University, P.O. Box 44, Sohar P.C. 311, Sultanate of Oman
S. N. Rashid ; University of Malaya Centre for Ionic Liquids (UMCiL), University of Malaya, Kuala Lumpur 50603, Malaysia
M. R. M. Nor orcid id orcid.org/0000-0002-6521-4603 ; Halal Research Group, Academy of Islamic Studies, University of Malaya, 50603, Kuala Lumpur, Malaysi
M. Y. Zulkifli ; Halal Research Group, Academy of Islamic Studies, University of Malaya, 50603, Kuala Lumpur, Malaysi
Y. Alias ; c)cUniversity of Malaya Centre for Ionic Liquids (UMCiL), University of Malaya, Kuala Lumpur 50603, Malaysia; f)Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
M. E. S. Mirghani ; a)International Institute for Halal Research and Training (INHART), International Islamic University Malaysia, Kuala Lumpur 50728, Malaysia; g)Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia, Ku


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Sažetak

This study presents the potential role of natural deep eutectic solvents (NADESs) in a lipase-catalyzed hydrolysis reaction as both a co-solvent in an aqueous solution and as a main solvent. Ammonium salts such as choline chloride (ChCl) were paired with different hydrogen bond donors such as glycerol and malonic acid and sugars (glucose, fructose and sucrose). The hydrolysis of p-nitrophenyl palmitate by six different lipases: lipase from porcine pancreas (PR), lipase from Candida rugosa (CR), Amano lipase PS, from Burkholderia cepacia (AM), lipase from Rhizopus niveus (RN), lipase acrylic resin from Candida antartica (ARC), lipase B Candida antartica immobilized on Immobead 150, recombinant from Aspergillus oryzae (CALB), were tested in five NADESs. The
results showed that NADES3 prepared from ChCl/sucrose was the most promising solvent as it enhanced the activities of both CALB and lipase from porcine pancreas to 355 % and 345 %. The kinetics investigation confirmed the higher catalytic efficiency (kcat/Km) of lipases in the 40 % of (NADES3) and compared with the aqueous form. The trend
achieved by NADES may be a promising approach for applications and further perspectives as genuinely green industrial solvents.







This work is licensed under a Creative Commons Attribution 4.0 International License.

Ključne riječi

biotransformation; biotechnology; ionic liquids; choline chloride; p-nitrophenyl palmitate; lipase B Candida antartica

Hrčak ID:

206732

URI

https://hrcak.srce.hr/206732

Datum izdavanja:

13.10.2018.

Posjeta: 1.743 *