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Evaluation of an Integrated Cell Culture-based and PCR Assay for Diagnosis of Genital Herpes in Women

Anna Majewska orcid id orcid.org/0000-0001-9036-7452 ; Department of Medical Microbiology, Medical University of Warsaw, 5 Chalubinskiego Str, 02-004 Warsaw, Poland
Maciej Przybylski ; Department of Medical Microbiology, Medical University of Warsaw, 5 Chalubinskiego Str, 02-004 Warsaw, Poland
Tomasz Dzieciatkowski ; Department of Medical Microbiology, Medical University of Warsaw, 5 Chalubinskiego Str, 02-004 Warsaw, Poland
Ewa Romejko-Wolniewicz ; 1st Department of Obstetrics and Gynecology, Medical University of Warsaw, Starynkiewicza Sq. 1/3, 02–015 Warsaw, Poland
Julia Zaręba-Szczudlik ; 1st Department of Obstetrics and Gynecology, Medical University of Warsaw, Starynkiewicza Sq. 1/3, 02–015 Warsaw, Poland
Grazyna Mlynarczyk ; Department of Medical Microbiology, Medical University of Warsaw, 5 Chalubinskiego Str, 02-004 Warsaw, Poland


Puni tekst: engleski PDF 257 Kb

str. 206-206

preuzimanja: 350

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Sažetak

Diagnosis of genital herpes requires a combination of clinical presentation and laboratory studies. Laboratory diagnostics allow us to clearly establish the etiology (HSV-1 or HSV-2) in order to determine the course of infection and prognosis. Decisive factors in the selection of the appropriate test are: diagnostic goals, patient population, specimen type, and implementation of conditions for the specific method. In total, 187 samples collected during a routine gynecological examination from 120 women were examined for the presence of HSV-1 and HSV-2 in the genital area. Two methods were used to test swabs: cell culture isolation and PCR. HSV-1 was the dominant type of virus in both study groups. The cytopathic effect was observed in 67 (35.8%) cultures with clinical material. HSV-1 and HSV-2 DNA were detected by PCR in 73 (39.0%) cell cultures infected with clinical samples. We did not observe typical, virus related cytopathic changes in 13.7% DNA HSV positive cell cultures, but on the other hand we did not detect viral DNA in 6% of positive cell cultures. High values of the parameters, defining the usefulness of diagnostic tests (sensitivity, specificity, and predictive values) in both groups, are determined by previous viral replication in cell culture.

 

Ključne riječi

cell culture; cytopathic effect; diagnosis; genital herpes; HSV; PCR

Hrčak ID:

207006

URI

https://hrcak.srce.hr/207006

Datum izdavanja:

22.10.2018.

Posjeta: 766 *