Izvorni znanstveni članak
https://doi.org/10.15255/CABEQ.2018.1391
Immobilization of Glucose Oxidase on Eupergit C: Impact of Aeration, Kinetic and Operational Stability Studies of Free and Immobilized Enzyme
D. Pečar
orcid.org/0000-0001-7326-2261
; University of Maribor, Faculty of Chemistry and Chemical Engineering, Smetanova 17, SI-2000 Maribor, Slovenia
Đ. Vasić-Rački
orcid.org/0000-0003-3927-8264
; University of Zagreb, Faculty of Chemical Engineering and Technology, Savska c. 16, HR-10000 Zagreb, Croatia
A. Vrsalović Presečki
orcid.org/0000-0002-0247-4483
; University of Zagreb, Faculty of Chemical Engineering and Technology, Savska c. 16, HR-10000 Zagreb, Croatia
Sažetak
The effect of aeration on the stability of glucose oxidase in the reaction of glucose oxidation to gluconic acid was investigated by determining the operational stability decay rate constant in the process conditions. Eupergit C as a porous carrier was chosen for
the enzyme immobilization. To evaluate glucose oxidase operational stability during process conditions, experiments of glucose oxidation were carried out in the repetitive batch reactor with and without continuous aeration at different aeration levels. It was found that the decay rate of the free enzyme linearly depended on the air flow rate. Immobilization of glucose oxidase on Eupergit C significantly enhanced enzyme stability at higher aeration rates. Kinetics of the free and immobilized enzyme was also determined. The mathematical model of glucose oxidation catalysed by free and immobilized glucose
oxidase in the batch reactor was developed.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Ključne riječi
glucose oxidase; immobilization; oxygen mass transfer; enzyme stability; kinetics; mathematical modelling
Hrčak ID:
215717
URI
Datum izdavanja:
15.1.2019.
Posjeta: 999 *