Izvorni znanstveni članak
https://doi.org/10.2478/aiht-2019-70-3215
Effects of simvastatin and fenofibrate on butyrylcholinesterase activity in the brain, plasma, and liver of normolipidemic and hyperlipidemic rats
Antonija Vukšić
; University of Zagreb, School of Medicine, Department of Pharmacology, Zagreb, Croatia
Jasna Lovrić
; University of Zagreb, School of Medicine, Department of Medical Chemistry, Biochemistry, and Clinical Chemistry, Zagreb, Croatia
Paško Konjevoda
; Ruđer Bošković Institute, NMR Centre, Zagreb, Croatia
Nina Blažević
; University of Zagreb, School of Medicine, Department of Pharmacology, Zagreb, Croatia
Marinko Bilušić
; Polyclinic Bonifarm, Department of Clinical Pharmacology and Toxicology, Zagreb, Croatia
Vlasta Bradamante
; University of Zagreb, School of Medicine, Department of Pharmacology, Zagreb, Croatia
Sažetak
The study objective was to test the hypothesis that simvastatin and fenofibrate should cause an increase in butyrylcholinesterase (BuChE) activity not only in the plasma and liver but also in the brain of normolipidemic and hyperlipidemic rats. Catalytic enzyme activity was measured using acetylthiocholine (ATCh) and butyrylthiocholine (BTCh) as substrates. Normolipidemic and hyperlipidemic rats were divided in four groups receiving 50 mg/kg of simvastatin a day or 30 mg/kg of fenofibrate a day for three weeks and three control groups receiving saline. Simvastatin and fenofibrate caused an increase in brain BuChE activity in both normo- and hyperlipidemic rats regardless of the substrate. The increase with BTCh as substrate was significant and practically the same in normolipidemic and hyperlipidemic rats after simvastatin treatment (14–17% vs controls). Simvastatin and fenofibrate also increased liver and plasma BuChE activity in both normolipidemic and hyperlipidemic rats regardless of the substrate. In most cases the increase was significant. Considering the important role of BuChE in cholinergic transmission as well as its pharmacological function, it is necessary to continue investigations of the effects of lipid-lowering drugs on BuChE activity.
Ključne riječi
acetylthiocoline; butyrylthiocholine; lipid-lowering drugs; Wistar rats; Zucker rats
Hrčak ID:
218043
URI
Datum izdavanja:
21.3.2019.
Posjeta: 2.055 *