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https://doi.org/10.2478/acph-2020-0019

Potential antidiabetic and antioxidant activities of a heliangolide sesquiterpene lactone isolated from Helianthus annuus L. Leaves

SAMUEL O. ONOJA ; Department of Veterinary Physiology and Pharmacology, Michael Okpara University of Agriculture, Umudike, PMB 7267 Umuahia, Nigeria
CHARLES O. NNADI ; Department of Pharmaceutical and Medicinal Chemistry, University of Nigeria Nsukka, 410001 Enugu State, Nigeria
SAMUEL C. UDEM ; Department of Pharmaceutical and Medicinal Chemistry, University of Nigeria Nsukka, 410001 Enugu State, Nigeria
ARUH O. ANAGA ; Department of Veterinary Physiology and Pharmacology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, 410001 Enugu State, Nigeria


Puni tekst: engleski pdf 802 Kb

str. 215-226

preuzimanja: 788

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Sažetak

Heliangolide is a naturally occurring sesquiterpene lactone and its derivatives are biologically active compounds present in most medicinal plants. This study evaluated the antioxidant and antidiabetic properties of a heliangolide sesquiterpene lactone isolated from Helianthus annuus L. leaves. The heliangolide sesquiterpene lactone was isolated through combination of solvent-solvent partitioning, column chromatography, thin layer chromatography and high performance-liquid chromatography techniques. The antioxidant activity of the compound was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and nitric oxide radical scavenging assays while the antidiabetic effects were investigated in alloxan-induced diabetic rats. The heliangolide derivative at 954.2 µmol L–1 showed 23.7 % DPPH and 26 % nitric oxide radical inhibitions compared with 96.6 and 50.9 %, resp., displayed by the controls (2,271.2 µmol L–1). It also reduced the fasting blood glucose (FBG) levels in a time-dependent manner. The highest activity was recorded within 6 h post-treatment at 0.2 mmol kg–1 bm. The heliangolide derivative exhibited significant (p < 0.05) antioxidant and antidiabetic properties and provides basis for further development of constituents of Helianthus annuus leaves for the management of such diseases.

Ključne riječi

Helianthus annuus; heliangolide derivative; antidiabetic; antioxidant

Hrčak ID:

221294

URI

https://hrcak.srce.hr/221294

Datum izdavanja:

30.6.2020.

Posjeta: 1.823 *