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Croatian Medical Journal, Vol. 59 No. 2, 2018.

Izvorni znanstveni članak

https://doi.org/10.3325/cmj.2018.59.46

Suppression of the interleukin- 1ß-induced inflammatory response of human Chang liver cells by acute and subacute exposure to alcohol: an in vitro study

Katharina Mörs ; Department of Trauma, Hand and Reconstructive Surgery, Goethe-Reconstructive Surgery, Goethe-Reconstructive Surgery, Goethe-University, Frankfurt am Main Germany
Shinwan Kany ; Department of Trauma, Hand and Reconstructive Surgery, Goethe-Reconstructive Surgery, Goethe-Reconstructive Surgery, Goethe-University, Frankfurt am Main Germany
Jason-Alexander Hörauf ; Department of Trauma, Hand and Reconstructive Surgery, Goethe-Reconstructive Surgery, Goethe-Reconstructive Surgery, Goethe-University, Frankfurt am Main Germany
Nils Wagner ; Department of Trauma, Hand and Reconstructive Surgery, Goethe-Reconstructive Surgery, Goethe-Reconstructive Surgery, Goethe-University, Frankfurt am Main Germany
Claudia Neunaber ; Department of Trauma Surgery, Medical School Hannover,Hannover, Germany
Mario Perl ; Department of Trauma Surgery Trauma Center Murnau, Murnau,Germany
Borna Relja orcid id orcid.org/0000-0002-5625-8823 ; Department of Trauma, Hand and Reconstructive Surgery, Goethe-Reconstructive Surgery, Goethe-Reconstructive Surgery, Goethe-University, Frankfurt am Main Germany

Puni tekst: engleski pdf 549 Kb

str. 46-55

preuzimanja: 253

citiraj

Sažetak

Aim To evaluate protective immunosuppressive dose and
time-dependent effects of ethanol in an in vitro model of
acute inflammation in human Chang liver cells.
Method The study was performed in 2016 and 2017 in the
research laboratory of the Department of Trauma, Hand
and Reconstructive Surgery, the University Hospital of the
Goethe-University Frankfurt. Chang liver cells were stimulated
with either interleukin (IL)-1β or IL-6 and subsequently
treated with low-dose ethanol (85 mmol/L) or high-dose
ethanol (170 mmol/L) for one hour (acute exposure) or 72
hours (subacute exposure). IL-6 and IL-1β release were determined
by enzyme-linked immunosorbent assay. Neutrophil
adhesion to Chang liver monolayers, production
of reactive oxygen species, and apoptosis or necrosis were
analyzed.
Results Contrary to high-dose ethanol, acute low-dose
ethanol exposure significantly reduced IL-1β-induced IL-6
and IL-6-induced IL-1β release (P < 0.05). Subacute ethanol
exposure did not change proinflammatory cytokine
release. Acute low-dose ethanol exposure significantly
decreased inflammation-induced formation of reactive
oxygen species (P < 0.05) and significantly improved cell
survival (P < 0.05). Neither acute nor subacute high-dose
ethanol exposure significantly changed inflammationinduced
changes in reactive oxygen species or survival.
Acute and subacute ethanol exposure, independently of
the dose, significantly decreased neutrophil adhesion to
inflamed Chang liver cells (P < 0.05).
Conclusion Acute treatment of inflamed Chang liver cells
with ethanol showed its immunosuppressive potential.
However, the observed effects were limited to low-dose
setting, indicating the relevance of ethanol dose in the
modulation of inflammatory cell response.

Ključne riječi

Hrčak ID:

225593

URI

https://hrcak.srce.hr/225593

Datum izdavanja:

15.4.2018.

Posjeta: 763 *